| Project/Area Number |
05670244
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
寄生虫学(含医用動物学)
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| Research Institution | National Institute of Health |
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Principal Investigator |
KANAZAWA Tamotsu Chief of laboratory, Department ofParasitology, National Institute of Health, 寄生動物部, 室長 (10194888)
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| Co-Investigator(Kenkyū-buntansha) |
ASAHI Hiroko Superior-investigator, Department of Parasitology, National Institute of Health, 寄生動物部, 主任研究官 (90231109)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1994: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1993: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Echinococcus multilocularis / Macrophage / nitrogen intermediates / IFN-gamma / T_h 1 type T cell |
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| Research Abstract |
Echinococcus multilocularis infection attracts a lot numbers of macrophages at the infection site in mice. Activated macrophages by IFN-gamma kill the protpscoleces in vitro. However, in vivo, alveolar hydatid disease develop and mice finaly died. Our question was why macrophages are not able to protect against Echinococcus multilocularis infection. We got some answers about the above question through these experiments supported by this grant.
1) Phagocytosis of mouse peritoneal macrophages was suppressed 6 weeks after infection. This suppression was recovered by IFN-gamma.
2) Production of nitrogen intermediates from mouse peritoneal macrophages was not induced by echinococcus infection. However, IFN-gamma induced nitrogen intermediates production of macrophages recovered from infected mice.
3) Macrophages recovered from infected mice did not kill protoscoleces in vitro. IFN-gamma made these macrophages kill protoscoleces in vitro.
4) The IFN-gamma production from infected mice were probably suppressed due to suppression of T_H 1 type T cell.
It remains to be clarified why T_h1type T cells are suppressed by echinococcus infection.
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