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Studies on macrophages recoverd from Echinococcus multilocularis infected mouse.

Research Project

Project/Area Number 05670244
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 寄生虫学(含医用動物学)
Research InstitutionNational Institute of Health

Principal Investigator

KANAZAWA Tamotsu  Chief of laboratory, Department ofParasitology, National Institute of Health, 寄生動物部, 室長 (10194888)

Co-Investigator(Kenkyū-buntansha) ASAHI Hiroko  Superior-investigator, Department of Parasitology, National Institute of Health, 寄生動物部, 主任研究官 (90231109)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1994: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1993: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsEchinococcus multilocularis / Macrophage / nitrogen intermediates / IFN-gamma / T_h 1 type T cell
Research Abstract

Echinococcus multilocularis infection attracts a lot numbers of macrophages at the infection site in mice. Activated macrophages by IFN-gamma kill the protpscoleces in vitro. However, in vivo, alveolar hydatid disease develop and mice finaly died. Our question was why macrophages are not able to protect against Echinococcus multilocularis infection. We got some answers about the above question through these experiments supported by this grant.
1) Phagocytosis of mouse peritoneal macrophages was suppressed 6 weeks after infection. This suppression was recovered by IFN-gamma.
2) Production of nitrogen intermediates from mouse peritoneal macrophages was not induced by echinococcus infection. However, IFN-gamma induced nitrogen intermediates production of macrophages recovered from infected mice.
3) Macrophages recovered from infected mice did not kill protoscoleces in vitro. IFN-gamma made these macrophages kill protoscoleces in vitro.
4) The IFN-gamma production from infected mice were probably suppressed due to suppression of T_H 1 type T cell.
It remains to be clarified why T_h1type T cells are suppressed by echinococcus infection.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] T.KANAZAWA: "Arginine-depenen generation of reactive nitrogen inter-mediates is instrumental in the in vitro killing of protp-scoleces of Echinococcus multilocularis by activated macrophages" Parasite immunology. 15. 619-623 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] TAMOTSU KANAZAWA: "Effects of mebendazole and albendazole on secondary alveolar hydatid disease in Mongolian gerbils with special reference to the timing of treatment" Japanese Journal of Parasitology. 43. 305-307 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] H.Asahi: "Continuous cultivation of intraerythrocytic Plasmodium falciparum in a serum-free medium with the use of a growth-promoting factor" Parasitology. 109. 397-402 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kanazawa T.: "Arginine-dependent generation of reactive nitrogen intermediates is instrumental in the in vitro killing of protoscoleces of Echinococcus multilocularis by activated macrophage" Parasite Immunology. 15. 619-623 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kanazawa T.: "Effects of mebendazole and zlbendazole on secondary alveolar hydatid disease in Mongolian gerbils with special reference to the timing of treatment" Japanese Journal of Parasitology. 43. 305-307 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Asahi H.: "Continuous cultivation of intraerythrocytic Plasmodium falciparum in a serum-free medium with the use of a growthpromoting factor" Parasitology. 109. 397-402 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.KANAZAWA 他: "Arginine-dependent generation of reactive nitrogen intermediates is instrumental in the……" Parasite Immunology. 15. 619-623 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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