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Molecular Evolution of Major Histocompatibility Complex and T Cell Receptor Genes

Research Project

Project/Area Number 05670291
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Immunology
Research InstitutionHokkaido University

Principal Investigator

KASAHARA Masanori  Hokkaido University School of Medicine Department of Biochemistry Associate Professor, 医学部, 助教授 (30241318)

Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1993: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsMajor histocompatibility complex / Molecular evolution / Proteasome / Agnathan / 軟骨魚類 / 両生類 / アフリカツメガエル
Research Abstract

The specific aims of this project were i) to clarify the phylogenetic origin of the major histocompatibility complex (MHC) by cloning MHC genes of lower vertebrates, and ii) to identify general rules that govern the long-term evolution of the MHC.As to the first specific aim, we demonstrated that the cartilaginous fish has typical, polymorphic MHC genes.However, all attempts to isolate MHC genes from cyclostomes were unsuccessful, suggestimg that these animals might not have bona fide MHC genes.As to the second specific aim, we identified the following three rules that apply to MHC genes in general.First, overall domain organization of MHC genes is very well conserved in evolution despite the fact that the sequence conservation is poor.Second, the number of functionally important, polymorphic MHC genes shows little interspecies variation and falls between one and three per haplotype.Third, essential features of MHC polymorphism are well conserved in evolution.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (52 results)

All Other

All Publications (52 results)

  • [Publications] Flajnik,M.F.: "A Novel type of class I gene organization in vertebrates: A large family of non-MHC-linked class I genes is expressed at the RNA level in the amphibian Xenoupus." EMBO J.12. 4385-4396 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sato,K.: "Evolution of the MHC: Isolation of class II β-chain cDNA clones from the amphibian Xenopus laevis." J.Immunol.150. 2831-2843 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Greenberg,A.,: "Isolation of a shark immunoglobulin light chain cDNA clone encoding a protein resembling mammalian χ light chains: Implications for the evolution of light chains." Proc. Natl. Acad. Sci. U.S.A.90. 10603-10607 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kasahara,M.: "The evolutionary origin of the major histocompatibility complex: Polymorphism of class II α-chain genes in the cartilaginous fish." Eur. J. Immunol.23. 2160-2165 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shum,B.P.: "Isolation of a classical MHC class I cDNA from an amphibian: Evidence for only one class I locus in the Xenopus MHC." J. Immunol.151. 5376-5386 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 小針布実子: "TAP遺伝子の構造と機能" Medical immunology. 26. 283-289 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Salter-Cid.: "HSP70 genes are linked to THE Xenopus major histocompatibility complex." Immunogenetics. 39. 1-7 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kato,S.: "Isolation of Xenopus complement factor B complementary DNA and linkage of the gene to the frog MHC." J.Immunol.153. 4546-4557 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kasahara,M.: "Molecular evolution of the major histocompatibility complex." Dev. Comp. Immunol.18. 1 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 笠原正典: "MHCクラスI分子による抗原提示の分子機構" RADIOISOTOPES. 43. 323-324 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 笠原正典: "MHC遺伝子の起源と進化" 蛋白質 核酸 酵素. 40. 2606-2618 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kasahara,M.: "Mapping of acidic epididymal glycoprotein (Aeg) genes to mouse chromosome 17." Mammalian Genome. 6. 51-53 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kandil,E.: "Structural and phylogenetic analysis of the MHC class I-like Fc receptor gene." J. Immunol.154(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Noguchi,M.: "The MHC class I-like ZN-α2-glycoprotein gene maps to mouse chromosome 5." Immunogenetics. 42(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kasahara,M.: "Evolution of the major histocompatibility complex: A current overview." Transplant immunol.3. 1-20 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Flajnik, M.F.: "A novel type of class I gene organization in vertebrates : A large family of non-MHC -linked class I genes is expressed at the RNA level in the amphibian Xenopus." EMBO J.12. 4385-4396 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sato, K.: "Evolution of the MHC : Isolation of class II beta-chain cDNA clones from the amphibian Xenopus laevis." J.Immunol.150. 2831-2843 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Greenberg, A.: "Isolation of a shark immunoglobulin light chain cDNA clone encoding a protein resembling mammalian x light chains : Implications for the evolution of light chains." Proc.Natl.Acad.Sci.U.S.A.90. 10603-10607 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kasahara, M.: "The evolutionary origin of the major histocompatibility complex : Polymorphism of class II alpha-chain genes in the cartilaginous fish." Eur.J.Immunol.23. 2160-2165 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shum, B.P.: "Isolation of a classical MHC class I cDNA from an amphibian : Evidence for only one class I locus in the Xenopus MHC." J.Immunol.151. 5376-5386 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kobari, F.: "Structure and function of TAP genes." Medical Immunology.26. 283-289 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Salter-Cid.: "Hsp70 genes are linked to the Xenopus major histocompatibility complex." Immunogenetics. 39. 1-7 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kato, S.: "Isolation of Xenopus complement factor B complementary DNA and linkage of the gene to the frog MHC." J.Immunol.153. 4546-4557 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kasahara, M.: "Molecular evolution of the major histocompatibility complex" Dev.Comp.Immunol.18. 1 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kasahara, M.: "Molecular mechanism of antigen presentation by MHC I class molecules." RADIOISOTOPES. 43. 323-324 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kasahara, M.: "Origin and evolution of MHC genes." Proteins-Nucleic Acids-Enzymes. 40. 2606-2618 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kasahara, M.: "Mapping of acidic epididymal glycoprotein (Aeg) genes to mouse chromosome 17." Mammalian Genome. 6. 51-53 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kandil, E.: "Structural and phylogenetic analysis of the MHC class I-like Fc reportor gene." J.Immunol.154 (in presss). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Noguchi, M.: "The MHC class I-like Zn-alpha2-glycoprotein gene maps to mouse chromosome 5." Immunogenetics. 42 (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kasahara, M.: "Evolution of the major histocompatibility complex : A current overview" Transplant Immunol.3. 1-20 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Flajnik,M.F.: "A novel type of class I gene organization in vertebrates:A large family of non-MHC-linked class I genes is expressed at the RNA level in the amphibian Xenopus." EMBO J.12. 4385-4396 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Sato,K.: "Evolution of the MHC:Isolation of class II β-chain _cDNA clones from the amphibian Xenopus laevis." J.Immunol.150. 2831-2843 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Greenberg,A.,: "Isolation of a shark immunoglobulin light chain _cDNA clone encoding a protein resembling mammalian κ light chains:Implications for the evolution of light chains." Proc.Natl.Acad.Sci.U.S.A.90. 10603-10607 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kasahara,M.: "The evolutionary origin of the major histocompatibility complex:Polymorphism of class II α-chain genes in the cartilaginous fish." Eur.J.Immunol.23. 2160-2165 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Shum,B.P.: "Isolation of a classical MHC class I _cDNA from an amphibian:Evidence for only one class I locus in the Xenopus MHC." J.Immunol.151. 5376-5386 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] 小針布実子: "TAP遺伝子の構造と機能" Medical Immunology. 26. 283-289 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Salter-Cid.: "Hsp 70 genes are linked to the Xenopus major histocompatibility complex." Immunogenetics. 39. 1-7 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kato,S.: "Isolation of Xenopus complement factor B complementary DNA and linkage of the gene to the frog MHC." J.Immunol.153. 4546-4557 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kasahara,M.: "Molecular evolution of the major histocompatibility complex." Dev.Comp.Immunol.18. 1 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 笠原正典: "MHCクラスI分子による抗原提示の分子機構" RADIOISOTOPES. 43. 323-324 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 笠原正典: "MHC遺伝子の起源と進化" 蛋白質 核酸 酵素. 40. 2606-2618 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kasahara,M.: "Mapping of acidic epididymal glycoprotein (Aeg) genes to mouse chromosome 17." Mammalian Genome. 6. 51-53 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kandil,E.: "Structural and phylogenetic analysis of the MHC class I-like Fc receptor gene." J.Immunol.154(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Noguchi,M.: "The MHC class I-like Zn-α2-glycoprotein gene maps to mouse chromosome 5." Immunogenetics. 42(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kasahara,M.: "Evolution of the major histocompatibility complex:A current overview" Transplant Immunol.3. 1-20 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Flajnik,M.F.: "A novel type of class I gene organization in vertebrates:A large family of non-MHC-linked class I genes is expressed at the RNA level in the amphibian Xenopus." EMBO J.12. 4385-4396 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Sato,K.: "Evolution of the MHC:Isolation of class II β -chain cDNA clones from the amphibian Xenopus laevis." J.Immunol.150. 2831-2843 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Greenberg,A.: "Isolation of a shark immunoglobulin light chain cDNA clone encoding a protein resembling mammalian k light chains:Implications for the evolution of light chains." Proc.Natl.Acad.Sci.U.S.A.90. 10603-10607 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kasahara,M.: "The evolutionary origin of the major histocompatibility complex:Polymorphism of class II α-chain genes in the cartilaginous fish." Eur.J.Immunol.23. 2160-2165 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Shum,B.P.: "Isolation of classical MHC class I cDNA from an amphibian:Evidence for only one class I locus in the Xenopus MHC." J.Immunol.151. 5376-5386 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Salter-Cid,L.: "HSP70 genes are linked to the Xenopus major histocompatibility complex." Immunogenetics. 39. 1-7 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 小針 布実子: "TAP遺伝子の構造と機能" Medical Immunology. 26. 283-289 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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