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The study of virus DNA polymorphisms and its application to paternity testing

Research Project

Project/Area Number 05670400
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Legal medicine
Research InstitutionJuntento University School of Medicine

Principal Investigator

KOBAYASHI Ryo  Juntendo University School of Medicine, Research Associate, 医学部, 助手 (40146775)

Co-Investigator(Kenkyū-buntansha) ITOH Yukio  Juntendo University School of Medicine, Assistant Professor, 医学部, 講師 (70053345)
Project Period (FY) 1993 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1994: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1993: ¥900,000 (Direct Cost: ¥900,000)
Keywordspersonal identification / paternity testing / Epstein-Barr virus / short tandem repeat / DNA / PCR / polymorphism / polymorphism / 扁桃腺 / in situ hybridization
Research Abstract

In this project, we have tried to find the DNA markers that might be acquired in persons after their birth and be named acquired DNA markers. We have also tried to apply them for personal identification and paternity testing. In 1993, the third complementarity-determining region (CDR3) of immunoglobulin heavy chain gene was selected as one of acquired DNA markers, because this gene was rearranged during B-cell differentiation. However, the life span of the mature B-cells whose immunoglobulin gene was rearranged is generally considered to be about 2 weeks. Therefore, we did not think that this gene was the acquired DNA marker that could identify individuals. In 1994, we selected virus genes, especially Epstein-Barr (EB) virus as the acquired DNA marker, because this virus is a human herpesvirus that is carried by more than 90% of human population worldwide. We could detect the EB virus DNA in 92% of 98 DNA samples from peripheral blood lymphocytes using PCR that amplified BamW region of EB virus. EB virus DNA might be the acquired DNA marker, because this virus infects persons after their birth, and could be easily differentiable from the host DNA.In 1995, we focused the polymorphism of EB virus. EB virus has several short tandem repeat sequences and it is known that part of these sequences sometimes mutate during asymptomatic infection. So, we amplified the region of short tandem repeats of EB virus in the DNA isolated from peripheral blood lymphocytes using PCR and could observe the polymorphisms of EB virus in individuals. We concluded that EB virus might be considered as one of acquired DNA markers. However, we don't know how many strains of EB virus there are in persons, or how long EB virus is infected. These subjects will be had to elucidated.

Report

(4 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • Research Products

    (39 results)

All Other

All Publications (39 results)

  • [Publications] Mosier D.E.: "Epstein-Barr virus-associated human B-cell lymphomas in human PBL-reconstituted SCID mice" Epstein-Barr Virus and Associated Diseases. 225. 405-410 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kobayashi R.: "Autoantigen inhibits apoptosis of a human B cell leukemia that produces pathogenic rheumatoid factor" Journal of Immunology. 151. 7273-7283 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Itoh Y.: "A comparative analysis of nasal secretions from subjects with and without acute rhinitis and its application to forensic medicine" Japan Journal of Legal Medicine. 47. 85-92 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Matsuzawa S.: "Rapid detection of human seminal plasma proteins by membrane aspiration test (MAT)" Forensic Science International. 64. 119-124 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Iwayama S.: "Intracellular localization and DNA-binding activity of a class of viral early phosphoproteins in human fibroblasts infected with human cytomegalovirus (Towne strain)" Journal of General Virology. 75. 3309-3318 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takeuchi H.: "Highly sensitive detection of latent infection by Epstein-Barr virus in peripheral blood cells of healthy individuals and in non-neoplastic tonsillar tissues from tonsillitis patient by reverse transcription-polymerase chain reaction" Journal of Virological Methods. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kobayashi R.: "Design of novel oligonucleotide probes for sex determination and its forensic application" Advances in Forensic Haemogenetics. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Itoh Y.: "Evaluation of primer extension preamplification (PEP) in forensic studies" Advances in Forensic Haemogenetics. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sekizawa A.: "Prenatal diagnosis of Duchenne muscular dystrophy using a single fetal nucleated erythrocyte in maternal blood" Neurology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hasegawa M.: "Nasopharyngeal carcinoma and Epstein-Barr virus infection in Japanese patients" American Journal of Rhinology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 小林 了: "シングルローカスプローブを用いた個人識別成績の標準化について" DNA多型. 1. 117-121 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 小林 了: "性別鑑定のためのオリゴプローブの開発" DNA多型. 2. 206-211 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 小林 了: "亜急性硬化性全脳炎における麻疹ウイルスの持続感染" 日本臨床免疫学会会誌. 17. 776-779 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 小林 了: "ウイルスゲノムを使った個人識別" DNA多型. 3. 288-291 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 小林 了: "SSPE新潟1株の脳内持続感染による遺伝子変異の進展" 神経免疫学. 3. 126-127 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mosier, D.E.: "Epstein-Barr virus-associated human B-cell lymphomas in human PBL-reconstituted SCID mice" Epstein-Barr Virus and Associated Diseases. 225. 405-410 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kobayashi, R.: "Autoantigen inhibits apoptosis of a human B cell leukemia that produces pathogenic rheumatoid factor" Journal of Immunology. 151. 7273-7283 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Itoh, Y.: "A comparataive analysis of nasal secretions from subjects with and without acute rhinitis and its application to forensic medicine" Japan Journal of Legal Medicine. 47. 85-92 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Matsuzawa, S.: "Rapid detection of human seminal plasma proteins by membrane aspiration test (MAT)" Forensic Science Internatnional. 64. 119-124 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Iwayama, S.: "Intracellular localization and DNA-binding activity of a class of viral early phosphoproteins in human fibroblasts infected with human cytomegalovirus (Towne strain)" Journal of General Virology. 75. 3309-3318 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takeuchi, H.: "Highly sensitive detection of latent infection by Epstein-Barr virus in peripheral blood cells of healthy individuals and in non-neoplastic tonsillar tissues from tonsillitis patient by reverse transcription-polymerase chain reaction" Journal of Virological Methods. (In press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kobayashi, R.: "Design of novel oligonucleotide probes for sex determination and its forensic application" Advances in Forensic Haemogenetics. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Itoh, Y.: "Evaluation of primer extension preamplification (PEP) in forensic studies" Advances in Forensic Haemogenetics. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sekizawa, A.: "Prenatal diagnosis of Duchenne muscular dystrophy using a single fetal nucleated erythrocyte in maternal blood" Neurology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hasegawa, M.: "Nasopharyngeal carcinoma and Epstein-Barr virus infection in Japanese patients" American Journal of Rhinology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hiroaki Takeuchi: "Hughly sensitive detection of latent infection by Epstein-Barr virus in peripheral blood cells of healthy individuals and in non-neoplastic tonsillar tissues from tonsillitis patient by reverse transcription-polymerase chain reaction" J Virol Methods. (in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] Ryo Kobayashi: "Design of novel oligonucleotide probes for sex determination and its forensic application" Adv Forensic Haemogenrtics. (in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] Yukio Itoh: "Evaluation of primer extension preamplification (PEP) in forensic studies" Adv Forensic Haemogenetics. (in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] Makoto Hasegawa: "Nasopharyngeal carcinoma and Epstein-Barr virus infection in Japanese patients" Am J Rhinology. (in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] 小林 了: "ウイルスゲノムを使った個人識別" DNA多型. 3. 288-291 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 小林 了: "SSPE新潟1株の脳内持続感染による遺伝子変異の進展" 神経免疫学. 3. 126-127 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Satoshi Iwayama: "Intracellular localization and DNA-biding activity of a class of viral early phosphoproteins in human fibroblasts infected with human cytomegalovirus (Towne strain)" J Gen Virol. 75. 3309-3318 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ryo Kobayashi: "Detection of Epstein-Barr virus infection in the tonsils by in situ hybridization and RT-PCR" Epstein-Barr Virus and Associated Disease. 89- (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 小林了: "性別鑑定のためのオリゴプローブの開発" DNA多型. 2. 206-211 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 小林了: "亜急性硬化性全脳炎における麻疹ウイルスの持続感染" 日本臨床免疫学会会誌. 17. 776-779 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yukio Itoh: "A comparative analysis of nasal secretions from subjects with and without acuto rhinitis and its application to forensic medicine" Jpn J Legal Med. 47. 85-92 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Donald E.Mosier: "Epstein-Barr virus-associated human B-cell lymphomas in human PBL-reconstituted SCID mice" Epstein-Barr Virus and Associated Disease. 225. 405-410 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ryo Kobayashi: "Autoantigen inhibits apoptosis of a human B cell leukemia that produces pathogenic rheumatoid factor" J Immunol. 151. 7273-7283 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 小林 了: "シングルローカスプローブを用いた個人識別成績の標準化について" DNA多型. 1. 117-121 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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