| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1993: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Research Abstract |
In order to analyze the antemortem pathophysiological state in cases of sudden unexpected death (SUD), particularly of sudden cardiac death, we examined clinical laboratory parameters in cadaver blood obtained from autopsy cases at Department of Legal Medicine, the Jikei University School of Medicine. As a trial for the pathogenetic analysis of SUD in young persons with marked coronary arteriosclerosis, we identified cholesteryl ester transfer protein (CETP) gene mutations in cadaver blood by means of PCR method. Furthermore, we analyzed mitochondrial DNA (mDNA) mutations in cardiac muscle from the cases of sudden cardiac death. As results, serum concentration of LDH,CPK,GOT,GPT and myoglobin in cadaver blood was so abnormally increased as a postmortem changes that these parameters were not valuable for diagnosis of early myocardial ischemia. Concerning the CETP gene analysis, neither homozygote nor heterozygote was detected in 40 cases of marked coronary arteriosclerosis, indicating that high density lipoprotein cholesterol (HDL-C) may not so markedly increased in our cases. Furthermore, we found deletion or mutation of myocardial mDNA in our cases, regardless of sudden cardiac death. However, there was no tendency of deleted mDNA frequency against sudden cardiac death.
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