| Project/Area Number |
05670425
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
内科学一般
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| Research Institution | KYUSHU UNIVERSIT |
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Principal Investigator |
SINGU Masao Kyushu University Medical Institute of Bioregulation Associate Professor, 生体防御医学研究所, 助教授 (10038523)
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| Co-Investigator(Kenkyū-buntansha) |
EZAKI Ichiko Kyushu University Medical Institute of Bioregulatio Lecturer, 生体防御医学研究所, 助手 (40037453)
末永 康夫 九州大学, 生体防御医学研究所, 助手 (20264111)
和田 哲也 九州大学, 生体防御医学研究所, 助手 (30220958)
野中 史郎 九州大学, 生体防御医学研究所, 助手 (60198603)
橋本 通 九州大学, 生体防御医学研究所, 助手 (00237950)
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| Project Period (FY) |
1993 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1993: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | Rheumatoid arthristis / Osteoclast / Osteoblast / Adhesion molecule / ICAM-1 / Type I collagen fragment / ICAM-1単クローン抗体 / IL-1β / IL-1beta |
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| Research Abstract |
The adhesion molecules such as ICAM-1, VCAM-1, ELAM-1 and ICAVM-1, LA-4 in human osteoblasts(OB) and osteoclastic cells(OC), respectively, obtained from rhheumatoid arthristis(RA)patients were expressed and enhanced by inflammatory cytokines.OB/OC interaction mediated by such adhesion molecules induced the suppression of osteoplastic properties of OB,leading to bone destruction and bone resorption. The bone destruction was mediated by metalloproteases, cytokines and oxygen free radicals produced by OC.There were no differences of bone resorbin activity between RA OC and osteoarthristis OC.Some synovial cells obatained from RA showed bone resorbing activity. The measurement of type I collagen fragment by specifice ELISA well reflected bone resoprtion, and more sensitive than pit assay. OC or OB cultured with antiICAM-l monoclonal antibody(mab)showed enhanced expression of VCAM-1 and ELAM-1, suggesting that there are some adhesion molecule cascades like in endothelial cells. Accordingly, ICAM-1 mab did not suppress arthristis in CII-mediated artghritis in mice. OC from RA patients showed increased bone resobing activity, where as frequency of TRAP staining was not increased.
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