| Project/Area Number |
05670429
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
内科学一般
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| Research Institution | Jichi Medical School |
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Principal Investigator |
TAKEDA Akira Jichi Med.School Department of Medicine, Assist.Prof., 医学部, 講師 (90155002)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1993: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Autoimmunity / Sjogren Syndrome / HHV-6 / HHV-7 / T cell clone / シェーグレン症候群 |
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| Research Abstract |
This research project was aimed to define the role of HHV-6 and HHV-7 in the pathogenesis of Sjogren syndrome especially from the point of view of virus specific cellular immune responses.
1 We have isolated salivary glands-infiltrating T lymphocytes from minor salivary glands obtained by lip biopsy from the patients with Sjogren syndrome, and expanded them in vitro with IL2 to be rendered to the examination for the HHV-6 or HHV-7 specific T cell proliferative responses. Autologous PBMC which were sensitized with sonicated antigens made from HHV-infected CBMC (cord blood mononuclear cells) were used to stimulate those T cells. We found neither responsiveness of the T cells from minor salivary glands to the HHV-6 nor HHV-7 antigens.
2 We have obtained sera from the patients with Sjogren syndrome and the normal individuals to examine for the titers of the antibodies to HHV-6 and HHV-7 by the immunofluorescence assay using virus-infected CBMC as well as the ELISA we have developed. The levels of the antibodies to HHV-6 and HHV-7 in the sera from Sjogren patients were found to be higher than those in normal individuals.
The way we used in this wotk provided a unique approach to the detection or identification of the immunopathological antigens involved in the pathogenesis of autoimmune diseases. This work showed the absence of HHV-6 and HHV-7 specific T cell immune responses in the local development of the lesion in salivary glands of Sjogren patients although the high levels of antibodies to the HHV-6 and HHV-7 in the patients suggested some pathological link between the disease and the infection with those viruses in terms of the systemic humoral immunity.
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