| Project/Area Number |
05670452
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Gastroenterology
|
|---|
| Research Institution | University of Tokyo |
|---|
Principal Investigator |
SHIRATORI Yasushi University of Tokyo, Dept.Int.Med., Assistant, 医学部(病), 助手 (70196624)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KOMATSU Yukata University of Tokyo, Dept.Int.Med., 医学部(病), 医員
MATSUMURA Masayuki University of Tokyo, Dept.Int.Med., 医学部(病), 医員
NIWA Yasuro University of Tokyo, Dept.Int.Med., 医学部(病), 医員
丹羽 康郎 東京大学, 医学部(病), 医員
|
|---|
| Project Period (FY) |
1993 – 1994
|
| Project Status |
Completed (Fiscal Year 1994)
|
| Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1994: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1993: ¥1,500,000 (Direct Cost: ¥1,500,000)
|
|---|
| Keywords | hepatic sinusoid / endothelial cells / Kupffer cells / liver-associated NK cells / TGF-alpha / CINK / IL-2 / molecular technique / 肝内皮細胞 / TGFd / PKC / CINC / BRM / Kupffer細胞 / 接着因子 |
|---|
| Research Abstract |
Liver lobule consists of parenchymal cells and four types of sinusoidal cells. Establishment and maintenance of hepatic sinusoidal endothelial cells have been investigated. Kupffer cells release several types of growth factors for hepatic endothelial cells in vitro, and some of them are TGF-alpha. During proliferation of the sisnuoidal endothelail cells, PKC expression increased in the cells. In addition, extracellular matrix modulated morphological changes of hepatic endothelial cells during angiogenesis.
Liver-associated NK cells play an important role in immune defense system in the liver, which has been found to be increased by interleukin 2 produced by macrophages upon stimulation of biologically responsive mediators. tn enhanced transcription level of IL-2 in macrophages has been demonstrated by PCR.
In addition, infiltrating of neutrophils has been analyzed by measuring the transcription level or protein production of interleukin-8, cytokine-induced neutrophil chemoattractant (CINK) in rodent. Production of this mediator has been also modulated by cytokine network in the liver as well.
Present study clearly demonstrated cellular communication in the liver by molecular techniques.
|
