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Biological activity of the ANAE-1 derived from the granuloma macrophage

Research Project

Project/Area Number 05670531
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Respiratory organ internal medicine
Research InstitutionOita Medical University

Principal Investigator

TSUDA Tomiyasu  Oita Medical University Faculty of Medicine, professor, 医学部, 教授 (80040567)

Co-Investigator(Kenkyū-buntansha) MIYAZAKI Eishi  Oita Medical University Faculty of Medicine, Research Associate, 医学部, 助手 (00264333)
YUBISUI Toshitsugu  Kochi Uiversity, professor, 理学部, 教授 (00019564)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1994: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1993: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsANAE-1 / MIF / epitherioid cell granuloma / ANAE-1 / マクロフアージ遊走阻止活性
Research Abstract

We purified the ANAE-1 from adjuvant-induced rabbit lung granuloma for analyzing the biological function of it. We homogenized granulomatous tissue resected from the lungs of rabbit that had received an Freund's complete adjuvant injection 4 weeks before. The ANAE-1 was purified as a single band by SDS-PAGE with standard method of chlumn chromatography. The purified ANAE-1 have showed macrophage migration inhibition activity over 80 nM.We tried to analyze the amino acid sequence of the ANAE-1 for molecular analysis. But we do not success because the ANAE-1 is not a single protein by isoelectric focusing.
On the other hand, when we examined whether human epithelioid cells have a specific ANAE-isozyme as well as rabbit epithelioid cells have the ANAE-1, any specific isozymes were observed in human epithelioid cells compared with human alveolar macrophages.

Report

(3 results)
  • 1995 Final Research Report Summary
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Yubisui Toshitugu: "An inflame deletion of codon 298 of the NADH-cytochrome b5 reductase gene results in herediatry methemoglobinemia." J. Biol. Chem.269. 5952-5957 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yubisui Toshitugu: "Cloning and nucleotide sequnece to a cDNA of the human reticulocyte NADPH- flavin reductase." BBRC. 198. 1170-1176 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsuda Tomiyasu: "Identification of IL-1 inhibitory activity as IL-1 receptor antagonist (IL-1ra) in vitroand linetics of Il-1ra and IL-1β in experimental rabbit lung granuloma." Experimental Lung Research 21. 21. 309-329 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Miyazaki Eishi: "A case of Acute Eosinophilic Pneumonia Caused by Inhalation of Trichosporon Terrestre." Am. J. Respir. Crit. Care. Med.151. 541-543 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsuda Tomiyasu: "Charactarization of distribution of T lymphocyte subsets and activated T lymphocytes infiltrating into sarcoid lesions." Internal Medecine. 34. 847-855 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yubisui Toshitugu: "An inflame deletion of codon 298 of the NADH-cytochrome b5 reductase gene results in herediatry methemoglobInemia." J.Biol. Chem.269. 5952-5957 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yubisui Toshitugu: "Cloning and nucleotide sequnece to a cDNA of the human reticulocyte NADPH-flavin reductase." BBRC. 198. 1170-1176 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsuda Tomiyasu: "Identification of IL-1 inhibitory activity as IL-1 receptor antagonist (IL-1ra) in vitro and kinetics of IL-1ra and IL-1 beta in experimental rabbit lung granuloma." Experimental Lung Research. 21. 309-329 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Miyazaki Eishi: "A case of Acute Eosinophilic Pneumonia Caused by Inhalation of Trichosporon Terrestre." Am. J.Respir. Crit. Care. Med.20GD04 : 151. 541-543 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsuda Tomiyasu: "Charactarization of distribution of T lymphocyte subsets and activated T lymphocytes infiltrating into sarcoid lesions." Internal Medicine. 34. 847-855 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yubisui Toshitsugu: "An inflame deletion of codon 298 of the NADH-cytochrome b5 reductase gene results in hereditary methemoglobinemia." J.Biol.Chem.269. 5952-5957 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yubisui Toshitsugu: "Cloning and nucleotide segunece of a cDNA of the human reticulocyte NADPH-flavin reductose" BBRC. 198. 1170-1176 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yubisui Toshitsugu: "The structure of human erythrocyte NADH-cytochrome b5 reductase at 2.5A resolution." J.Mol.Biol.(発表予定). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Tsuda Tomiyasu: "Identification of IL-1 inhibitory activity AS IL-1 receptor Antagonist(IL-Ira)in vitro and kinetics of IL-Ira and IL-IBn." Experimental Lung Research. 21. 309-329 (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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