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The effect of platelet activating factor on mucociliary regulation in airway epithelium cultured at airinterface

Research Project

Project/Area Number 05670538
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Respiratory organ internal medicine
Research InstitutionTokyo Women's Medical College

Principal Investigator

KONDO Mitsuko  Tokyo Women's Medical College 1st Dep.of Medicine, Instructor, 医学部, 助手 (50178430)

Co-Investigator(Kenkyū-buntansha) KONDO Mitsuko  Tokyo Women's Medical College 1st Dep.of Medicine, Instructor (50178430)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1993: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordsplatelet activating factor / ion transport / calcium ion / inositol triphosphate / prostaglandin / air interface / airway epithelial cells / プロスタグランデイン / air interface / カルシウム
Research Abstract

To elucidate the effect of platelet activating factor on mucociliary transport of airway epithelial cells, we studied bioelectric properties of cow tracheal epithelial cells cultured at an airinterface. PAF dose-dependently increased short-circuit current and conductance. This effect was inhibited by Cl channel blocker, DPAC,but not Na channel blocker, amiloride. In addition, this effect was inhibited by the pretreatment of PAF antagonist, BAPTA-AM,and indomethacin, suggesting that PAF increases Cl secretion via PAF receptor, calcium and prostaglandin dependent pathway. Therefore, to elucidate the signal transduction of PAF in the airway epithelial cells, we measured second messengers.
In fura2-loaded cells, PAF increased [Ca^<2+>]i in a dose-dependent manner. This [Ca^<2+>]i response consisted of a transient increase and a subsequent sustained elevation. Ca^<2+>-free medium totally abolished the sustained response, but it only inhibited the transient response. Moreover, PAF increased inositol triphosphate level whose time course was similar to the transient calcium response. PAF also increased PGE2 and PGF2alpha by RIA assay.
These results suggest that PAF-induced epithelial function is mediated by the increased calcium and prostaglandins.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (5 results)

All Other

All Publications (5 results)

  • [Publications] 近藤光子: "気道粘膜における細胞間相互作用-上皮イオントランスポートに対する好酸球の影響-" 日本胸部疾患学会雑誌. 32. 1067-1072 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M. Kondo: "The effect of platelet activating factor on intracellular free calcium in cow tracheal epithelium" American Journal of Respiratory Cell and Molecular Bilogy. 10. 279-283 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Kondo et al: "Cell-to Cell Interaction in Airway Mucosa-Effect of Eosinophils on Airway Epithelial Ion Transport" The Japanese Joural of Thoracic Diseases. 32. 1067-1072 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Kondo et al: "The effect of platelet activating factor on intracellular free calcium in cow tracheal epithelium" American Journal of Respiratory Cell and Molecular Biology. 10. 279-283 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Kondo: "The effect of platelet activating factor on intracellular free calcium in cow tracheal epithelium" Am.J.Respir.Cell Mol.Biol. 10(in press). (1994)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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