| Project/Area Number |
05670542
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | University of Occupational and Environmental Health |
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Principal Investigator |
NAGATA Shoji Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health Professor, 産業生態科学研究所, 教授 (10108722)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIKAWA Toshio National Center of Psychiatry and Neurology, Kohnodai Hospital Division of Psych, 神経センター・国府台病院・心身総合診療科, 医長 (90136375)
IRIE Masahiro Institute of Industrial Ecological Sciences Assistant lecturer, 産業生態科学研究所, 助手 (00248593)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1994: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1993: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Conditioning / Anaphylaxis / Histamine / Substance P / Guinea Pig / Central nervous system / Respiratory resistance / Asthma / conditicning / SubstanceP / Central neruaus system |
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| Research Abstract |
The involvement of central nervous system (CNS) in bronchial hypereactivity which is characteristic feature of bronchial asthma was investigated.
1.Results of the study about the effect of several antagonists to adrenergic, cholinergic and tachykinin receptor on conditioned histamine release suggested that substance P (SP) in sensory nerve may have an important role in this reaction. An increare of percent eosinophil in bronchoalevelar lavage fluid (BALF) and the number of eosinophils in peribronchial and peribronchiolar tissues was observed 6 hours after exposure of conditional stimulus.
2.Many SP immunoreactive nerve fivers were found under the epitheluim of bronchus or around the blood vessels and bronchioles in the study of immunofluerescence micrographs. Some figures of mast cells which released histamine and macrophages which phagocyted histamine and SP in lysozome were observed after the injection of caspsaicin (2mg/kg) by immunoelectron microscopy study.
3.A novel tachykinin antagonist FK224 showed 74.5% inbition of increased respiratory resistance after fild stimulation of nonadrenergic noncholinergic nerve and 77.7% inhibition after injection of SP,suggesting that FK224 which was used in the inhibition study of conditioned histamine release, may work as an effective antagonist of SP.
4.Anterior hypothalamic lesions in sensitized guinea pig inhibited not only bronchoconstriction after antigen challenge, but suppressed the late allergic reaction indicated by increased percent of eosinoplils in BALF and number of infiltrated eosinoplils around bronchi and bronchioles.
These results indicated that the CNS can influence both immediate and late allergic reaction in the respiratory system, sensory neuropeptides including SP may have an important role in the regulation of these reactions.
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