| Project/Area Number |
05670543
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | TOKYO METROPOLITAN INSTITUTE OF GERONTOLOGY |
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Principal Investigator |
KIDA Kozui TOKYO METROPOLITAN INSTITUTE OF GERONTOLOGY,DEPARTMENT OF IMMUNOPATHOLOGY,RESEARCH SCIENTIST., 免疫病理部門, 研究員 (90142645)
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| Co-Investigator(Kenkyū-buntansha) |
NOMURA Koichiro TOKYO METROPOLITAN INSTITUTE OF GERONTOLOGY,DEPARTMENT OF IMMUNOPATHOLOGY,RESEAR, 免疫病理部門, 研究員
OGATA Megumi TOKYO METROPOLITAN INSTITUTE OF GERONTOLOGY,DEPARTMENT OF IMMUNOPATHOLOGY,RESEAR, 免疫病理部門, 研究員
MIZUUCHI Tomoko TOKYO METROPOLITAN INSTITUTE OF GERONTOLOGY,DEPARTMENT OF IMMUNOPATHOLOGY,RESEAR, 臨床病理部門, 研究員
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | beta-AMINOPROPIONITRILE / GROWING RAT / ALVEOLAR FORMATION / CAPILLARY CONFIGURATION / EXTRACELLULAR MATRIX / LUNG GROWTH AND DEVELOPMENT / beta-aminopropionctrile / 幼老ラット |
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| Research Abstract |
beta-AMINOPROPIONITRILE (betaAPN), A LATHYROGEN THAT INHIBITS LYSYL OXIDASE ACTIVITY,WAS REPEATEDLY ADMINISTERED TO MALE RAT PUPS FROM 2 DAYS OF AGE UNTIL SACRIFICE AT FOUR WEEKS OF AGE.BY SCANNING ELECTRON MICROSCOPY,THE LUNGS OF THE TREATED RATS SHOWED A CORROSION CAST OF ALVEOLAR CAPILLARIES THAT APPEARED ABNORMAL IN THICKNESS AND CONFIGURATION ; TRANSMISSION ELECTRON MICROSCOPY SHOWED EXTENSIVE MORPHOLOGICAL CHANGES IN EXTRACELLULAR MATRIC (ECM), INCLUDING COLLAGEN, ELASTIN,GLYCOSAMINOGLYCAN AND INTERSTITIAL CELLS.QUANTITATIVE ANALYSIS REVEALED AN INCREASE IN GLYCOSAMINOGLYCAN OF UP TO 24.4%, A DECREASE IN COLLAGEN (44.5%), AND THE DISAPPEARANCE OF LIPID-LADEN INTERSTITIAL CELLS : HOWEVER,THE CHANGE IN ELASTIN WAS LIMITED TO MORPHOLOGICAL APPEARANCE.FROM THESE OBSERVATIONS,WE CONCLUDE THAT ALTERATIONS IN CAPILLARY CONFIGURATION ARE CONNECTED TO EXTENSIVE CHANGES IN THE ECM,AND THESE CHANGES IN ECM COMPONENTS MIGHT ALSO BE INVOLVED IN THE EMPHYSEMA-LIKE LUNGS INDUCED BY betaAPN.
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