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Detection of cytokines in brain lesims of humans and animal model.

Research Project

Project/Area Number 05670544
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Neurology
Research InstitutionTohoku University

Principal Investigator

NAKAMURA Shozo  Tohoku University, Neurology, Associate Prof., 医学部付属病院・神内, 講師 (80108498)

Co-Investigator(Kenkyū-buntansha) SEKIZAWA Tsuyoshi  Tohoku University, Neurology, Assistant, 医学部付属病院・神内, 助手 (50150264)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1994: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1993: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordscytokine / immunocytochemistry / SSPE / PML / HAM / AIDS / Cytokine / Immnohistochemistry / immunohistochemistry
Research Abstract

We analyzed frozen brain tissues from three patients with subacute sclerosing panencephalitis (SSPE) and a patient with progressive multifocal leukoencephalopathy (PML) for the presence of IL-6, interferon-gamma, tumor necrosis factor (TNF) and transforming grwoth factor (TGF) -beta. We detected these cytokines in SSPE brain lesions showing extensive cellular infiltrates, demyelination, and gliosis. Moreover, we also detected IL-6, TNF-alpha, IFN-gamma and TGF-beta in brain lesions detected JC virus antigens from a patient with PML.These results indicate that IL-6, IL-1beta, IFN-gamma, TNF-alpha, and TGF-beta may be produced in SSPE and PML lesions, and may be involoved in the lesion pathogenesis of SSPE (Nagano I et al : Neurology 1994 ; 44 : 710-715).
We also reported that TNF-alpha-positive cells constituted 1.6-18% and 8.2-23.5% of the total number of cerebrospinal fluid cells from six of 12 patients with HTLV-1-associated myelopathy and in all samples obtained from inflammatory cases, respectively.
We examined the immunopathology and the expression of human immunodeficiency virus type 1 (HIV-1) in lumbosacral dorsal root ganglia (DRGs) from 16 patients with AIDS and 10 HIV-1-serological controls. Therefore, we concluded that low levels of replication of HIV-1, through cytotoxic T lymphocytes or expression of cytokines, may play a role in the subclinical degeneration of sensory neurons frequently observed in DRGs of AIDS patients.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Nakamura S.et al: "Detection of TNF-α-positive alls in CSF of patients with HAM" J.Neuroimmrnol. 42. 127-130 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 中村 正三 他: "中枢神経感染症における髄液中IFN-γおよびIL-6産生細胞の検索" 臨床神経. 32. 143-147 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 中村 正三 他: "脊髄症状を呈した髄膜癌腫症の1例-浸潤腫瘍細胞のTNF-αの検出" 臨床神経. 33. 182-186 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yoshioka M.et al: "Expression of HIV-1 and IL-6 in lumbosacral dorsal root ganglia of patients with AIDS" Neurology. 44. 1120-1130 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nagano I.et al: "Expression of cytokines in brain lesions in subacntic sclerosing panencephalitis" Neurology. 44. 710-715 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nakamura S.et al: "Detection of TNF-α positive tuma cells in patients with leptomeningeal carcimatosis" Acta Neurologica Scand.in press.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nakamura S et al: "Detection of tumor necrosis factor-alpha-positive cells in CSF of patients with HAM." J Neuroimmunol. 42. 127-130 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nagano I et al: "Immunocytochemical analysis of the cellular infiltrate in brain lesions in SSPE." Neurology. 41. 1639-1642 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nagano I et al: "Expression of cytokines in brain lesions in subacute sclerosing panencephalitis." Neurology. 44. 710-715 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yoshioka M et al: "Expression of HIV-1 and interleukin-6 in lumbosacral dorsal root ganglia of patients with AIDS." Neurology. 44. 1120-1130 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nakamura S et al: "Detection of IFN-gamma and IL-6 producing cells in CSF cells in CNS infectious diseases using immunocytochemistry." Clin Neurol. 32. 143-147 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nakamura S et al: "A case of meningeal carcinomatosis showing myelopathy-Detection of TNF-alpha in infiltrating CSF cells and brain tissue sections of cerebellum." Clin Neurol. 33. 182-186 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nakamura S et al: "Detection of TNF-alpha in infiltrating tumor cells in patients with leptomeningeal carcinomatosis." Acta Neurol Scandinav. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nakamura S.et al: "Detection of TNF-α-positive cells in CSF of patieits with HAM" J.Neuroimmurol. 42. 127-130 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] 中村 正三 他: "中枢神経感染症における髄液中IFN-γおよびいIL-6産生細胞の検索" 臨床神経. 32. 143-147 (1992)

    • Related Report
      1994 Annual Research Report
  • [Publications] 中村 正三 他: "脊髄症状を呈した髄膜癌腫症の1例-浸潤腫瘍細胞のTNF-αの検出" 臨床神経. 33. 182-186 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yoshioka M.et al: "Expression of HIV-1 and IL-6 in lumbosacral dorsal root ganglin of patieits with AIDS" Neurology. 44. 1120-1130 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Nagano I.et al: "Expression of Cytokines in brain lesions in subacute sclerosing panencephalitis" Neurology. 44. 710-715 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Nakamura S.et al: "Detection of TNF-α-positive tumor cells in potieits with lepto meningel carcimomatosis" Acta Neurologica Scand. (in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Nakamura S,et al: "Detection of TNF-α political cells in CgF of patients with HAM" J.Neuroimmunol. 42. 127-130 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 中村正三他: "中枢神経感染症における髄液中IFN-γおよびIL-6産生細胞の検索" 臨床神経. 32. 143-147 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 中村正三他: "脊髄症状を呈した髄膜癌腫症の1例-浸潤腫瘍細胞のTNF-αの検出" 臨床神経. 33. 182-186 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Nagano I,Nagamura S.: "Detection of several cytokine in SSPE" Neurology. (in press).

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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