| Project/Area Number |
05670559
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurology
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
MIMORI Yasuyo Hiroshima Univ.School of Med., Research Associate, 医学部, 助手 (50166112)
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| Co-Investigator(Kenkyū-buntansha) |
KATAYAMA Sadao Hiroshima Univ.Medical Hospital, Research Associate, 医学部・附属病院, 助手 (00211160)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1994: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1993: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | Alzheimer's disease / Parkinson's disease / beta-adrenergic receptor / Adenylate cyclase / Acetylcholinesterase / Brain microvessels / Arteriole / Capillary |
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| Research Abstract |
We studied the beta-adrenergic receptor-adenylate cyclase system in the intraparenchymal brain microvessels in the patients with Alzheimer's disease and control subjects. Micro-vessels were isolated from frozen autopsied cortex and subsequent separation of the arterioles from the capillary fraction was performed. In Alzheimer brain, beta-receptor density was significantly reduced in the arteriole fraction without changes in affinity, whereas the capillary fraction and whole homogenate did not show significant difference. Adenylate cyclase activity, either basal or stimulated, in both vessel fractions did not differ between controls and Alzheimer patients. Brain microvessels from patients with Parkinson's disease did not change in beta-receptor parameters. The activity together with properties of acetylcholinesterase (AChE) were also examined in microvessel preparations from either control or Alzheimer brain. The relative amount of asymmetric or nonextractable AChE was significantly higher in microvessels than in brain parenchyma, although AChE in micovessels consisted mainly of globular forms. The AChE activity either in the arteriole or the capillary fractions from Alzheimer patients was significantly higher than that of controls, while the parenchymal activity was much lowered in Alzheimer brain. In addition, the relative amount of asymmetric AChE in microvessels of Alzheimer brain was elevated significantly as compared to the control brain. These changes in receptor density as well as enzyme properties in brain microvessels are somewhat specific for diseases and may be related to the disease conditions and the symptoms in the neurodegenerative disorders.
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