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Angiotensin II receptor gene expression in the hypertensive endorgan damages and the effect of the treatment.

Research Project

Project/Area Number 05670617
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Circulatory organs internal medicine
Research InstitutionEhime University School of Medicine

Principal Investigator

KOHARA Katsuhiko  Ehime Univ 2nd Dept Intern Med, Lecturer, 医学部・附属病院, 講師 (30260384)

Co-Investigator(Kenkyū-buntansha) NISHIDA Wataru  School of Medicine, Ehime Univ.Assistant Professor, 医学部・附属病院, 助手 (80271089)
ABURAYA Masahito  School of Medicine, Ehime Univ.Assistant Professor, 医学部・附属病院, 助手 (00260688)
OKURA Takafumi  School of Medicine, Ehime Univ.Assistant Professor, 医学部・附属病院, 助手 (40260385)
Project Period (FY) 1993 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1995: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
KeywordsAngiotensin II / Receptor / AT1 receptor subtype / AT2 receptor / SHR / Left Ventricular Hypertrophy / AT1 receptor antagonist / ACE inhibitor / 副腎 / 脳組織 / 両側腎摘除 / アンジオテンシンII / AT_1受容体拮抗薬 / AT_1 / AT_2 / レニン・アンジオテンシン系 / in situ hybridization / WKY
Research Abstract

The levels of AT1A and AT1B receptor mRNA expression were evaluated by RT-PCR in spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats (WKY). At the age of 16-week old, there was no difference in AT1 receptor subtypes expression in the heart, adrenal gland or kidney between SHR and WKY except for the aorta in which AT1B was less expressed in SHR.
Antihypertensive treatment with either AT1 receptor antagonist or ACE inhibitor resulted in regression of left ventricular hypertrophy in SHR.After one week of the treatment, At1 receptor antagonist significantly decreased both AT1A and AT1B receptor mRNA expression, and ACE inhibitor significantly increased the levels of AT1A mRNA in the left ventricle of SHR.However, after four week of the treatment with both AT1 receptor antagonist and ACE inhibitor, the levels of AT1A and AT1B mRNA were returned to the control levels.
Using newly developed method, we evaluated the AT2 gene expression in rat adrenal gland. Bilateral nephrectomy as well as exogenous Ang II administration significantly increased the gene expression of AT2 mRNA in the rat adrenal gland. Althogh the property of AT2 receptor in the adrenal gland is still unknown, these findings indicate that alteration of the renin-angiotensin system can change the AT2 receptor gene expression.
Results obtained in the study indicate that Ang II receptor shows subtype specific changes in a tissue specific manner.

Report

(4 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Wakamiya R,Kitami Y Okura T,Kohara K,Hiwada K: "Effect of AT1 receptor blockade on expression of genes for type-1 angiotensin II (AT1) receptor subtypes in spontaneously hypertensive rats." Hypertension Research. 17. 187-192 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Rumi Wakamiya,Kohara,Kunio Hiwada: "Gene expression of the type-1 angiotensin II receptor in Katsuhiko rat adrenal gland." Blood Pressure. 3 (suppl5). 109-112 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Rumi Wakamiya, Yutaka Kitami, Takafumi Okura, Katsuhiko Kohara, Kunio Hiwada: "Effect of AT1 receptor blockade on expression of genes for type-1 angiotensin II (AT1) receptor subtypes in spontaneoulsy hypertensive rats." Hypertension Research. 17. 187-192 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Rumi Wakamiya, Katsuhiko Kohara, Kunio Hiwada: "Gene expression of the type-1 angiotensin II receptor in rat adrenal gland." Blood Pressure. 3 (Suppl 5). 109-112 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Wakamiya R et al.: "Regulation of the angiotensin II type-1 receptor gene expression in spontaneously hypertensive rats." Hypertension Research. 17. 187-192 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Wakamiya R et al.: "The gene expression of type 1 angiotensin II receptor in the rat adrenal gland." Blood Pressure. 3(suppl 5). 109-112 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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