| Project/Area Number |
05670618
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | KURUME UNIVERSITY (1994)
Kyushu University (1993) |
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Principal Investigator |
IMAIZUMI Tsutomu KURUME UNIVERSITY,MEDICINE,PROFESSOR & CHAIRMAN, 医学部, 教授 (60148947)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1993: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | Insulin / Vasodilation / Hypertension / Syndromex / インシュリン / ラット / 交感神経活動 / 脳室内投与 / 高血圧 |
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| Research Abstract |
we determined the role of endothelium in insulin-induced vasodilation in young healthy volunteers (n=13). Forearm blood flow (FBF) was measured by strain gauge plethysmography. A cannula was inserted in the brachial artery for direct measurements of arterial pressure and drug infusions. Intra-arterial infusion of insulin for 15 minutes increased plasma insulin level from 15.8 <plus-minus> 1.7 at rest to 141.6 <plus-minus> 12.3muU・mL^<-1> (p<.01). intra-arterial infusion of insulin (n=7) for 15 minutes increased FBF 4.8 <plus-minus> 1.4 at rest to 6.0 <plus-minus> 1.7mL・min^<-1>・100mL^<-1> and increased plasma 6-keto prostaglandin F_1alpha (PGF_1alpha), stable metabolite of prostacyclin, from 14.9 <plus-minus> 3.3 at rest to 29.3 <plus-minus> 5.7 pg/ml. Thus intra-arterial infusion of insulin caused forearm vasodilation with increases in prostacyclin in the venous effluents. Intra-arterial infusion of insulin was repeated after administration of indomethacin 75 mg per os (n=7). Indomethacin abolished insulin-induced vasodilation and increases in PGF_1alpha. In another group of subjects (n=6), intra-arterial infusion of insulin was repeated after intra-brachial artery infusion of N^G-monomethyl-L-arginine (L-NMMA,n=7) at 4.0mumol・min^<-1> for 5 minutes (n=6). Infusion of L-NMMA decreased resting FBF to 3.6 <plus-minus> 1.0mL・min^<-1>・100mL^<-1> (p<.05) without changes in resting blood pressure and heart rate. Infusion of L-NMMA did not affect insulin-induced forearm vasodilation.Our results may suggest that insulin-induced vasodilation is mediated by prostacyclin but not by nitric oxide in the forearm of heathy humans.
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