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Distribution and function of phosphoinositide second messenger system in developing brain

Research Project

Project/Area Number 05670659
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Pediatrics
Research InstitutionNational Institute of Neuroscience, NCNP

Principal Investigator

MIZUGUCHI Masashi  Department of Mental Retardation and Birth Defect Research, National Institute of Neuroscience, NCNP Section Chief, 神経研究所・疾病研究第2部, 室長 (20209753)

Co-Investigator(Kenkyū-buntansha) YAMADA Mitsunori  Department of Pathology, Brain Research Institute, Niigata University Assistant, 脳研究所・実験神経病理学部門, 助手 (30240039)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1993: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsSecond messenger / Inositol 1,4,5-trisphosphate / Cyclic ADP-ribose / CD38 / Calcium ion / Immunohistochemistry / Central nervous System / イノシトールリン脂質 / 細胞内情報伝達
Research Abstract

A.Studies on the phosphoinositide-mediated system.
Developmental changes of inositol 1,4,5-trisphosphate 3-kinase (IP3K) were investigated in rat cerebellum by immunoelectron microscopy. An important role of this system during development was suggested by the high expression of IP3K observed on structures having special developmental significance, such as the synapses between the prarllel fibers and the proximal dendrites of Purkinje cells.
B.Studies on the cyclic ADP-ribose (cADPR) -mediated system.
The expression in brain of CD38 antigen, which is involved in both the formation and hydrolysis of cADPR,was investigated with polyclonal antibodies raised against synthetic CD38 peptide fragments. Using these antibodies in addition to monoclonals, the presence of CD38 in brain was demonstrated by both immunochemical and immunohistochemical methods.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Yamada,M.et al: "Specific expression of inositol 1,4,5-trisphosphate 3-kinase in dendritic spines." Brain Research. 606. 335-340 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yamada,M.et al: "Developmental profile of inositol 1,4,5-trisphosphate 3-kinase in rat cerebellar cortex: light and electron microscopic immunohistochemical studies." Developmental Brain Research. 71. 137-145 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Mizuguchi,M.et al.: "Expression of Bcl-2 protein in murine neural cells in culture." Brain Research. 649. 197-202 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 水口 雅ら: "ラット脳におけるCD38免疫反応性" Neuropathology. 14. S212- (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Otsuka,N.et al: "CD38 immunoreactivity in Alzheimer's neurofibrillary tangles." Brain Pathology. 4. 558- (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 水口 雅ら: "ラット脳におけるCD38免疫反応性" Neuropathology. 14. S212 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Otsuka,N.et al.: "CD38 immunoreactivity in Alzheimer's neurofibrillary tangles." Brain Pathology. 4. 558 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yamada,M.et al.: "Specific expression of inositol 1,4,5-trisphosphate 3-kinase in the dendritic spines." Brain Pathology. 4. 383 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Mizuguchi,M.et al.: "Expression of Bcl-2 protein in murine neural cells in culture." Brain Research. 649. 197-202 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 水口 雅: "組織培養を用いた神経系の発生と老化に関する研究-ホスホリパーゼCアイソザイムの中枢神経系培養細胞における分布-" 笹川医学医療記念財団・海外派遣研究者報告. 21-25 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 山田光則: "Specific expression of inositol 1,4,5‐trisphosphate 3‐kinase in dendritic spines." Brain Research. 606. 335-340 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 山田光則: "Developmental profile of inositol 1,4,5‐trisphosphate 3‐kinase in rat cerebella cortex: light and electron microscopic immunohistochemical studies." Developmental Brain Research. 71. 137-145 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 水口 雅: "Infantile bilateral striatal necrosis: chronic and acute manifestations in a single case." Brain and Development. 16(印刷中). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 水口 雅: "Expression of Bcl‐2 protein in murine neural cells in culture." Brain Research. (印刷中). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 水口 雅: "先天性代謝異常症" 日本臨床. 51. 2919-2923 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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