• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Morphological analysis of cardiac malformations in developing WKY/NCrj rat embryos raised in whole embryo

Research Project

Project/Area Number 05670671
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Pediatrics
Research InstitutionShiga University of Medical Science

Principal Investigator

NAKAGAWA Masao  SUMS,Dept of Ped.Ass.Prof., 医学部, 講師 (40188909)

Co-Investigator(Kenkyū-buntansha) KURIBAYASHI Toshiro  KPUM,Dept of 2nd Anatomy, Ass.Prof., 医学部, 講師 (80186519)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1993: ¥700,000 (Direct Cost: ¥700,000)
KeywordsWhole embryo culture / Sixth aortic arch / HNK-1 / N-CAM / WKY / NCrj rat / Neural crest cell / cardiac anomaly / N-CAM
Research Abstract

1.Morphological analysis of the heart in WKY/NCrj and control rat embryos raised in whole embryo culture.
1) Sixth aortic arches were detected in both WKY/NCrj and control rat embryos cultured for 12 hours from 11.5 days of gestation. No morphological differences of the sixth aortic arches were observed between these two strains of embryos during 48 hours of culture.
2) As the embryos cultured over 48 hours showed developmental delay compared with age-matched controls, this whole embryo culture system appeared useful only when performed for 48 hours.
2.Immunolocalizations of extracellular molecules including migrating neural crest cell markers in the heart of WKY/NCrj and control rat embryos.
HNK-1, N-CAM,fibronectine and cytotactin were immunopositive in the sixth aortic arches of both WKY/NCrj and control rat embryos at 11.5,12.5 and 13.5 day of gestation. The distributional patterns of those markers were almost same between WKY/NCrj and control rat embryos. N-CAM showed a chain of immunopositive tissues from nerve fibers around the foregut to the truncus arteriosus via the sixth aortic arch, which indicated that the sixth aortic arch was one of the pathways neural crest cells migrate through from splanchnic mesenchymal tissues to the truncus arteriosus. However, this study also suggested that cardiac malformations in WKY/NCrj rat might not be induced by hypoplastic sixth aortic arches or abnormal migrations of neural crest cells through this pathway.
Conclusively, the hypoplastic sixth aortic arches in WKY/NCrj rat embryos at 14.5 day of gestation were supposed to be secondarily brought by an abnormal truncal division. Therefore, we are planning to clarify the distributional patterns or migratory pathways of cardiac neural crest cells which may play an important role in truncal division.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] Masao Nakagawa: "Developmental anatomy of HNK-1 immunoreactivity in the embryonic rat heart:co-distribution with early conduction tisuue" Anat. Embryol.187. 445-460 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 中川雅生: "心臓の発生におけるType-1 CollagenaseおよびlL-1αの発現とRemodeling" 心筋の構造と代謝-1993. 16. 369-376 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Masao Nakagawa: "Three-dimensional reconstruction of HNK-l immunoreactivity in the embryonic rat heart:codistribution with early cardiac conduction tissue." Developmentel mechanisms of heart disease (Clark EB, Markwald RR, Takao A eds,Futura, N.Y.). 327-332 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 中川雅男: "刺激伝導系の発生" Annual Review 循環器1995. 160-165 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Thomas K. Borg: "Extracellular matrix, receptors, and heart development." Developmental mechanisms of heart disease (Clark EB, Markwald PR, Takao A eds, Futura N.Y.). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hiroto Okagawa: "Immunolocalization of N-CAM in the heart of the early developing rat embryo." Anat Rec.(掲載予定).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 奥野昌彦: "ウイルス性心筋炎モデルマウスにおけるサイトカインの発現において" 心筋の構造と代謝-1994. 17(掲載予定). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Toshiro Kuribayashi: "Persistent common atrioventricular canal of the complete form associated wiht anomalies of the aortic arch system inWKY/NCRJ rat fetuses." Jpn Circ J.58. 720-726 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Masao Nakagawa: "Developmental anatomy of HNK-1 immunoreactivity in the embry-onic rat heart : co-distribution with early conduction tissue." Anat Embryol. 187. 445-460 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Masao Nakagawa: "Expression of type-1 collagenase and IL-1 alpha in developing rat heart." Cardiac Structure and Metabolism. 16. 369-376 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Masao Nakagawa: "Three-dimensional reconstruction of HNK-1 immunoreactivity in the embryonic rat heart : codistribution with early cardiac conduction tissue." Developmental mechanisms of heart disease. Clark EB,Markwald RR,Takao A.eds.Futura, N.Y. 327-332 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Masao Nakagawa: "Developmental anatomy of cardiac conduction tissue." Annual Review Cardiology 1995 Chugai-igakusya. 160-165 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Thomas K.: "Borg. Extracellular matrix, receptors, and heart development." Developmental mechanisms of heart disease. Clark EB,Markwald RR,Takao A.eds. Futura, N.Y.(1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hiroto Okagawa: "Immunolocalization of N-CAM in the heart of the early developing rat embryo." Anat Rec. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Toshiro Kuribayashi: "Persistent common atrioventricular canal of the complete form associated with anomalies of the aortic arch system in WKY/NCrj rat fetuses." Jpn Circ J. 58. 720-726 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Masahiko Okuno: "Expression of cytokines in the mouse heart of viral acute myocarditis." Cardiac Structure and Metabolism. 17 (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hiroto Okagawa: "Immunolocalization of N-CAM in the heart of the early developing rat embryo." Anatomical Record. (掲載予定).

    • Related Report
      1994 Annual Research Report
  • [Publications] 岡川浩人: "家族性原発性肺高血圧の1学童例" 心臓. 26. 639-644 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 成宮正朗: "ポンプ失調による心不全で発病した急性心筋炎の2乳児例" 心臓. 26. 981-986 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 笹原彰子: 心臓. 27(掲載予定). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 中川雅生: "心臓の発生におけるType-1 CollagenaseおよびIL-1αの発現とRemodeling" 心筋の構造と代謝-1993. 16. 369-376 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 奥野昌彦: "ウイルス性心筋炎モデルハウスにおけるサイトカインの発現について" 心筋の構造と代謝-1994. 17(掲載予定). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Masao Nakagawa: "Developmental mechanisms of heart disease" Clark EB,Markwald RR,Takao A(eds),Futura,New York, (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Thomas K.Borg: "Developmental Mechanisms of heart disease" Clark EB,Markwald RR,Takao A(eds),Futura,New York, (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 中川雅生: "Annual Review 循環器 1995" 杉本恒明、松本昭彦、杉下靖郎、門間和夫編、 中外医学社、東京, 298 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Masao Nakagawa,Robert P.Thompson,Louis Terracio,Thomas K.Borg: "Developmental anatomy of HNK-1 immunoreactivity in the embryonic rat heart:co-distribution with early conduction tissue" Anatomy and Embryology. 187. 445-460 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 中川雅生、Louis Terracio,Thomas K.Borg: "心臓の発生におけるType-1 CollagenaseおよびIL-1αの発現とRemodeling" 心筋の構造と代謝-1993. 16(掲載予定). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Masao Nakagawa,Robert P.Thompson,Thomas K.Borg,Louis Terracio: "Three-dimensional reconstruction of HNK-1 immunoreactivity in the embryonic rat heart:Co-distribution with early conduction system" Proccedings of the 4th international symposium on etiology and morphogenesis of congenital heart disease,Markwald,RR Clark,E Takao,A(eds)Futura,New York. (掲載予定). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Thomas K.Borg,Louis Terracio,Wayne Carver,Masao Nakagawa: "Extracellular matrix during development of the heart" Proccedings of the 4th international symposium on etiology and morphogenesis of congenital heart disease,Markwald,RR Clark,E Takao,A(eds)Futura,New York. (掲載予定). (1994)

    • Related Report
      1993 Annual Research Report

URL: 

Published: 1993-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi