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Studies of latent infection and reactivation of human cytomegalovirus

Research Project

Project/Area Number 05670684
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Pediatrics
Research InstitutionSapporo Medical University

Principal Investigator

NUMAZAKI Kei  Sapporo Medical University School of Medicine, Department of Pediatrics, Assistant Professor, 医学部, 講師 (00228272)

Co-Investigator(Kenkyū-buntansha) WAKAI Shuji  Sapporo Medical University School of Medicine, Department of Pediatrics, Instruc, 医学部, 助手 (30230907)
TSUTSUMI Hiroyuki  Sapporo Medical University School of Medicine, Department of Pediatrics, Assista, 医学部, 講師 (80217348)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1993: ¥800,000 (Direct Cost: ¥800,000)
Keywordshuman cytomegalovirus (HCMV) / polymerase chain reaction (PCR) / T cell subsets / cytokine / soluble interleukin 2 receptor (sIL-2R) / サイトカイン / ヒトサイトメガロウイルス / 潜伏感染 / CD_8陽性細胞
Research Abstract

We tried to detect human cytomegalovirus (HCMV) DNA in CD4+ and CD8+ T lymphocytes from fourteen infants with HCMV hepatitis by polymerase chain reaction (PCR) assay. HCMV was isolated from their urine and anti-HCMV IgM antibody was detected in their sera. One set of primers were designed from a region-a major immediate early (IE) gene. We detected HCMV IE DNA in the specimens obtained from six infants. HCMV IE DNA was detected from CD4+ cells in two cases and from DC8+ cells in one. In three cases, HCMV IE DNA was detected from both CD4+ and DC8+ cells. We also studied the relationship between HCMV infection and serum levels of cytokines. We determined serum levels of interleukin-4 (IL-4), tumor necrosis factor alpha (TNF-a) and soluble interleukin 2 receptor (sIL-2R) which associated with the activation of T lymphocytes by enzyme immunoassay. In the acute phase of HCMV infection, titers of sIL-2R were correlated with serum levels of liver enzymes. IL-4 and TNF-a activities were not detected in sera. We concluded that the acute phase of primary HCMV infection was associated with T lymphocytes. This study shows that IL-2 may play a significant role in the hepatic dysfunction associated with primary HCMV infection.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Numazaki K,Nagata N et al: "Effectofglycyrrhizin,cyclosporinA,and tumornecrosis factor α on infection of U-937 and MRC-5cells by human cytomegalovirus." JLeukocBiol. 55. 24-28 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Numasaki K,Umetsu M et al: "Effect of glycyrrhizin in children with liver dysfunction associated with cytomegalovirus infection" Tohoku J Exp Med. 172. 147-153 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Numazaki K et al: "Analysis of human cytomegalovirus-infected peripheral bloodmononuclear cells frominfants with liver dysfunction by flow cytometry〜" J Leukoc Biol. 56. 187-191 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Numazaki K,Chiba S et al: "Diagnostic value of rapid detection of Chlamydiatrachomatis〜" Diagn Microbiol Infect Dis. 17. 233-234 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Numazaki K,Chiba S et al: "Evaluation of diagnostic assays for neonatal and infantile chlamydialinfections." Tohoku J Exp Med. 170. 123-129 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Numazaki K,chiba S.: "Natural course and trial of treatment for infantile liver dysfunction associated with cytomegalovirus infections." In Vivo. 7. 477-780 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Numazaki K,Nagata N et al: "The First International Symposium of the Japan-Russia North East Asia Medical Exchange Proceedings" Japan Russia Medical Exchange Foundation, 321 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Numazaki K,Nagata N,Sato T,Chiba S.: "Effect of glycyrrhizin, cyclosporin A,and tumor necrosis factor alpha on infection of U-937 and MRC-5 cells by human cytomegalovirus." J Leukoc Biol. 55. 24-28 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Numazaki K,Umetsu M,Chiba S.: "Effect of glycyrrhizin in children with liver dysfunction associated with cytomegalovirus infection." Tohoku J Exp Med. 172. 147-153 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Numazaki K,Asanuma H,Nagata N,Chiba S.: "Analysis of human cytonegalovirus-infected peripheral blood mononuclear cells from infants with liver dysfunction by flow cytometry and the polymerase chain reaction." J Leukoc Biol. 56. 187-191 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Numazaki K,Chiba S.: "Diagnostic value of rapid detection of Chlamydia trachomatis by using amplified enzyme immunoassay in infants with respiratory infections." Diagn Microbiol Infect Dis. 17. 233-234 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Numazaki K,Chiba S,Niida Y,Komatsu M,Hashimoto N.: "Evaluation of diagnostic assays for neonatal and infantile chlamydial infections." Tohoku J Exp Med. 170. 123-129 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Numazaki K,Chiba S.: "Natural course and trial of treatment for infantile liver dysfunction associated with cytomegalovirus infections." In Vivo. 7. 477-780 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Numazaki K,Nagata N,Hotsubo T,Chiba S.: "Congenital and perinatal cytomegalovirus infection." Japan Russia Medical Exchange Foundation The First International Symposium of the Japan-Russia North East Asia Medical Exchange Proceedings. 321 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Numazaki K,nagata N et al: "Effect of glycyrrhizin,cyclosporin A, and tumor necrosis factor α on infection of U-937 and MRC-5 cells by human cytomegalovirus." J Leukoc Biol. 55. 24-28 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Numazaki K,Umetsu M et al: "Effect of glycyrrhizin in children with liver dysfunction associated with cytomegalovirus infection" Tohoku J Exp Med. 172. 147-153 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Numazaki K et al: "Analysis of human cytomegalovirus-infected peripheral blood mononuclear cells〜" J Leukoc Biol. 56. 187-191 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Numazaki K,Chiba S et al: "Diagnostic value of rapid detection of Chlamydiatrachomatis〜" Diagn Microbiol Infect Dis. 17. 233-234 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Numazaki K,Chiba S et al: "Evaluation of diagnostic assays for neonatal and infantile chlamydialinfections." Tohoku J Exp Med. 170. 123-129 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Numazaki K,Chiba S.: "Natural course and trial of treatment for infantile liver dysfunction associated with cytomegalovirus infections." In Vivo. 7. 477-780 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Numazaki K,Nagata N et al: "The First International Symposium of the Japan-Russia North East Asia Medical Exchange Proceedings" Japan Russia Medical Exchange Foundation, 321 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Numazaki,K.: "Natural course and trial of treatment for infantile liver dysfunction associated with cytomegalovirus infections" In Vivo. 7. 477-480 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Numazaki,K.: "Effect of glycyrrhizin,Cyclosporin A,and tumor necrosis factord on infection of U-937 and MRC-5 cells by human cytomegalovirus" Journal of Leukocyte Biology. 55. 24-28 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Numazaki,K.: "Effect of glycyrrhizin in children with liver dystunction asscciated with cytomegalovirus imfection" Tohoku Journal of Experimental Medicine. 172(印刷中). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Numazaki,K.: "The First International Symposium of the Japan-Russia Noth East Asia Medical Exchange,Proceedings" The Japan Russia Medical Exchange Foundation, 3 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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