| Project/Area Number |
05670700
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | Teikyo University School of Medicine |
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Principal Investigator |
ABE Toshiaki Teikyo Univ.Sch.Med., professor, 医学部, 教授 (40101117)
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| Co-Investigator(Kenkyū-buntansha) |
OGAWA Kiyoko (GOTO Kiyok) Teikyo Univ.Sch.Med., associate, 医学部, 助手 (50150660)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1994: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1993: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | cytomegalovirus / HNK-1 / sulphated glycosphingolipids / anti-receptor antibody / SGPG / SGLPG / 硫酸化糖鎖 / 抗体 |
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| Research Abstract |
In the present study we found that sera from patients with congenital CMV infection showed elevated titers* antibodies against sulfated glucuronyl glycosphingolipids (SGGL), which contain a monoclonal antibody HN* 1 reactive carbohydrate epitope. SGPG contains sulfated glucuronic acid on its non-reducing end of nLc4* and SGLPG has a similar structure but with an additional unit of lactosamine. Interesingly the antibodies* CMV infected patients'sera showed a higher reactivity to SGLPG than SGPG.This unique specificity * different from that of other HNK-1 related antibodies. Furthermore both anti-sulfatide and anti-SG* antibodies were absorbed with sulfatide-conjugated octyl-Sepharose column and heparin-Sepharose colur* whereas CMV specific IgG titer was not decreased by the absorption of anti-sulfated GL antibody. Th* results suggested that CMV infection might specifically induce production of antibodies against sulfated (* whereas these antibodies differed from CMV specific antibody.
Then we studied whether the antibodies were produced against altered glycoconjugate patterns of host cell* a result of CMV infection. It was found that purified HNK-1 antibody bound to HCMV-positive and -negat* antigens. Anti-SGGL antibodies, which were partially purified from patient's sera by affinity chromatograp* also bound to both antigens, whereas the reactivity was lower than that of anti-HCMV antibodies. These res* indicate that a level of the HNK-1 epitope expression was not altered by HCMV infection and also t* glycoconjugates containing HNK-1 epitope were present in HCMV permissive cells. Furthermore, * pretreatment of the purified HNK-1 antibody with HCMV permissive cells prior to virus inoculation inhibi* plaque formation of HCMV,suggesting that the glycoconjugates containing HNK-1 carbohydrate epitope in* host cell have a special role in the process of HCMV infection.
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