| Project/Area Number |
05670714
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | Kurume University |
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Principal Investigator |
SAKAGUCHI Hinako (1994) Kurume University Medicine Research Associate, 医学部, 助手 (50258422)
井上 治 (1993) 久留米大学, 医学部, 講師 (20193576)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIYORI Astushi Kurume University Medicine Research Associate, 医学部, 助手 (30218226)
KATO Hirohisa Kurume University Medicine Professor, 医学部, 教授 (30080724)
坂口 美奈子 久留米大学, 医学部, 助手 (50258422)
佐藤 登 久留米大学, 医学部, 助手 (50205952)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1993: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | kawasaki disease / Superantigen / TNF-alpha / 接着分子 / サイトカイン |
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| Research Abstract |
It has been recently reported that KD is associated with the selective expansion of vbeta2^+ and vbeta8.1^+ T cells in peripheral blood lymphocytes (PBL) by studying the T cell receptor (TCR) repertoire of in vitro activated T cells, and therefore, KD may be caused by a superantigen. To better understand immunopathology of KD,we have inestigated TCR Vbeta2 and Vbeta8.1 expression on both the T cells of freshly isolated PBL and T cell clones (TCC) from patients with KD.Cytokine production by TCC was also studied. Blood samples were obtained from patients with acute (n=20) and convalescent (n=20) KD,age-matched children with non-infectious diseases (n=18) and healthy adults (n=20). Among these four groups, there were no significant differences in the percentages of either Vbeta2^+ or Vbeta8.1^+ T cells of freshly isolated PBL.The same was true for the CD4^+ or CD8^+ T cell subsets. One hundred-five TCC (98 CD3^+ CD4^+ CD8^- and 7 CD3^+ CD4^- CD8^+) established from the affected skin, lymph node or PBL of six patients with KD were also negative for either Vbeta2 or Vbeta8.1 TCR.Sixty-eight of 105 TCC (65%) produced detectable levels (>5 pg per ml) of tumor necrosis factor-alpha (TNF-alpha) (6-1016 pg per ml), in the absence of any stimuli. In contrast, only 11 (10%) of 105 TCC or 7 (7%) of 97 TCC produced detectable levels of IL-2 or IL-6, respectively, in the absence of any stimuli. Stimulation with PHA and PMA induced the majority of TCC to produce higher amounts of TNF-alpha、IL-2 and IL-6.These results suggest that CD4^+ T helper cells expressing TCR-beta other than Vbeta2 or Vbeta8 receptor, primarily through TNF-alpha production, are involved in the immunopathology of KD.
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