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RESEARCH FOR TRANSMEMBRANE SIGNALING SYSTEM OF EPIDERMAL KERATINOCYTES ; INTRACELLULAR FREE CALCIUM AND PROTEIN KINASE C.

Research Project

Project/Area Number 05670717
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Dermatology
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

KOIZUMI Hiroko  HOKKAIDO UNIVERSITY MEDICAL HOSPITAL,DERMATOLOGY,LECTURER, 医学部附属病院, 講師 (60113552)

Project Period (FY) 1993 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1994: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1993: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsKERATINOCYTE / PROTEIN KINASE C / SPRR / PSORIASIS / CALCIUM / protein Kinase C / サブスタンスP / Keratinecyte / 魚鱗癬 / adenylate cyclase / substance P / 培養ヒト表皮角化細胞 / involucrin
Research Abstract

Epidermal keratinocytes proiferate and differentiate to make stratum corneum. Many chemical mediators, hormones and peptides stimulate keratinocytes to induce phosphophatidylinositol metablism, alteration of intracellular free calcium, adenylate cyclase response activation. We elucidated that bradykinin, thrombin, substance P,beta-adrenarin, adenosine, and histamine induce increase of intracellular free calcium. These effects are mediated with synthesis of inositol 1,4,5-trisphosphate or adenylate cyclase activation. The importance of calcium as a second messenger is shown. Presence of membrane skeleton proteins such as spectrin, b-fodrin, 4.1 protein like proteins were detected immunologically and the alteration of their distribution in cultured keratinocytes and diseased skin were observed. A new technique was reported to detect peroxide produced from keratinocytes using dihydrorhodamine 123. Differentiation associated expression of nPKC eta and SPRR was demonstrated in normal and diseased skin. The expression and cornified envelope formation are considered to mediated by activation of nPKC eta.
Cross-talk among transmembrane signaling systems in keratinocytes, and drugs to influence these systems should be investigated. Abnormal keratinization and accerelation of proliferation are considered to closely realt to pathogenesis of psoriasis. The alteration of membrane signaling systems in psoriasis and the action mechanisms of the drugs used in therapy of skin diseases are under investigation.

Report

(4 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • Research Products

    (67 results)

All Other

All Publications (67 results)

  • [Publications] Koizumi H.: "Differebtiation associated expression of nPKC in normal human skin and skin diseases." Journal of Investigative Dermatology. 101. 858-863 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Koizumi H.: "Clinical significance of serum adenosine deminase activity in patients with mycosis fungoides" Journal of Dermatology. 20. 394-399 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Koizumi H.: "Substance P induces inositol 1,4,5-trispnosphate and intracellular free calcium increase in cultured normal" human epidermal keratiocytes. Experimental Dermatology. 3. 40-44 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Osada S.: "Predominant expression of nPKC, a Ca^<2+> independent isoform of protein kinase C in epithelial tissue." Cell Growth and Differentiation. 4. 167-175 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 小泉洋子: "ケラチノサイトの増殖調節 情報伝達系" 日本皮膚科学会雑誌. 103. 16141-1620 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 小泉洋子: "プロテインカイネースC" 皮膚科の臨床. 35. 1297-1302 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shimizu T: "Immunoreaztive analogues of erythrocyte ankyrin in human epidermal keratinocyte" Arch Dermatol Res. ((in press))

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sato-Matsumura KC: "Localization of annexin I (lipocortin I, P35) mRNA in normal and diseased human skin by in situ hybridization" Arch Dermatol Res. ((in press))

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 小泉洋子: "神経ペプチドと皮膚アレルギー" 興和医報. 38. 644-646 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 小泉洋子: "皮膚の老化による表皮細胞膜情報伝達系の変化とその防止のための基礎的研究" 第4回資生堂皮膚老化研究ファンド研究報告書. 4. 2-5 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 小泉洋子: "ニューロペプチド" 皮膚科キーワード. ((印刷中))

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fujikawa K.: "Assignment of six patients with xeroderma pigmentosum in Hokkaido area to a variant form" J Radiat Res. 35. 168-178 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Koizumi H: "Dermal denderocyte hamartoma, -A novel connective tissue hamartoma" J Am Acad Dermato I. 32. 318-321 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takano J: "Ultraviolet action spectra for peroxide generation in human and pig epidermal keratinocytes loaded with dihydrorhodamine 123" Arch Dematol Res.287. 321-325 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shimizu T: "Localization of immuno-analogues of erythrocyte protein 4. 1 and spectrin in epidermis of psoriasis vulgaris" Histochemistry. 103. 363-368 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Koizumi H: "Differentiation associated localization of SPRR in normal and diseased skin." Br J Dematol. ((in press))

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Koizumi H: "Modulation of fransmembrane signaling system in senescence of human keratinocytes" Arch Dermatol Res. ((in press))

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shimizu T: "Nonerythoid membrane skeletal protein in normal and diseased human skin." Histology and Histopathology. ((in press))

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 小泉洋子: "ニューロペプタイド" 皮膚科の臨床. 35. 1325-1332 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sato KC: "A lichenoid skin lesion as a sign of β2-microglobulin induced amyloidosis in a long term haemodialysis patient." British journal of Dermatology. 128. 686-689 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 日野原重明: "今日の治療指針1993" 医学書院, 1304 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Koizumi H,Kohno Y,Ohkawar A,Kuroki T: "Differentiation associated expression of nPKCeta in normal human skin and skin diseases." Journal of Investigated Dermatology. 101. 858-863 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Koizumi H,Tomizawa K,Tanaka H,Kumikiri M,Ohkawara A: "Clinical significance of serum adenosine deaminase activity in patients with mycosis fungoides." Journal of Dermatology. 20. 394-399 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Koizumi H,Yasui C,Fukaya T,Ueda T,Ohkawara A: "Substance P induces inositol 1,4,5-trisphosphate and intracellular free calcium increase in cultured normal human epidermal keratinocytes." Experimental Dermatology. 3. 40-44 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Osada S,Hashimoto Y,Nomura S,Kohno Y,Chida K,Tajima O,Kubo K,Akimoto K,Koizumi H,Kitamura S,Suzuki K,Ohno S,Kuroki T: "Predominant expression of nPKCeta, a Ca2+independent isoform of protein kinase C in epithelial tissues, in association with epithelial differentiation." Cell Growth and Differentiation. 4 (3). 167-175 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Koizumi H: "signal transduction in epidermal keratinocytes" Japanese Journal of Dermatology. 103. 1614-1620 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Koizumi H: "Protein kinase C" Hihu Rinshou. 35. 1297-1302 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shimizu T,Takakuwa Y,Koizumi H,Ishibashi T,Ohkawara A: "Immunoreactive analogues of erythrocyte ankyrin in human epidermal keratinocyte." Archives of Dermatological Research. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sato-Matsumura KC., Koizumi H,Matsumura T,Ohkawara A,Takasu T,Furuta Y,Sawa H,Nagashima K: "Localization of annexin I (lipocortin I,P35) mRNA in normal and diseased human skin by in situ hybridization." Archives of Dermatological Research. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Koizumi H: "Neuropeptide and allergic skin diseases" Kowa Ihou. 38. 644-646 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Koizumi H: "Modulation of transmembrane signaling system in senescence of human keratinocytes" Shiseido Fund for Aging. 4. 2-5 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Koizumi H: "Neuropeptide" Hihu Keywords. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fujikawa K,Ayaki H,Ishizaki K,Taketera H,Matsuo S,Iizuka H,Koizumi, Ikenaga M: "Assigment of six patients with xeroderma pigmentosum in Hokkaido area to A variant form." J Radiat Res. 35. 168-178 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Koizumi H,Kumakiri M,Yamanaka K,Tomizawa K,Endo M,Ohkawara A: "Dermal denderocyte hamartoma, -A novel connective tissue hamartoma-" Journal of American Academy of Dermatology. 32 (2). 318-321 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takano J,Koizumi H,Ohkawara A,Kamo N,Ueda T: "Ultraviolet action spectra for peroxide generation in human and pig epidermal keratinocytes loaded with dihydrorhodamine 123." Archives of Dermatological Research. 287. 321-325 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shimizu T,Takakuwa Y,Koizumi H,Ishibashi T,Ohkawara A: "Localization of immuno-analogues of erythrocyte protein 4.1 and spectrin in epidermis of psoriasis vulgaris." Histochemistry. 103. 363-368 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Koizumi H,Tanaka H,Kartasova T,Ohkawara A,Kuroki T: "Differentiation associated localization of SPRR in normal and diseased skin. patterns of mouse sprI : correlation of expression with epithelial function." British Journal of Dermatology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Koizumi H,Ohkawara A: "Modulation of transmembrane signaling system in senescence of human keratinocytes" Archives of Dermatological Research. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shimizu T,Takakuwa Y,Koizumi H,Ohkawara A: "Nonerythroid membrane skeletal protein in normal and diseased human skin." Histology and Histopathology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Koizumi H: "Neuropeptide" Hihu Rinshou. 35. 1325-1332 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sato KC,Kumakiri M,Koizumi H,Ando M,Ohkawara A,Fujioka Y,Kon T: "A lichenoid skin lesion as a sign of beta2-microglobulin" British journal of Dermatology. 128. 686-689 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Koizumi H: "Dermal denderocyte hamartoma.‐A novel connective tissue hamartoma" J Am Acad Dermatol. 32. 318-321 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Takano J: "Ultraviolet action spectra for peroxide generation in human and pig epidermal keratinocytes loaded with dithdrothodamine 123" Arch Dermatol Res. 287. 321-325 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Shimizu T: "Localization of immuno‐analogues of erythrocyte protein 4.1 and spectrin in epidermis of psoriasis vuloaris" Histochemistry. 103. 363-368 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Koizumi H: "Differentiation associated locatization of SPRR in cormal and diseased skin." Br J Dermatol. in press.

    • Related Report
      1995 Annual Research Report
  • [Publications] Koizumi H: "Modulation of transmembrane signaling system in senescense of human kerationocytes" Arch Dermatol Res. in press.

    • Related Report
      1995 Annual Research Report
  • [Publications] Shimizu T: "Nonerythroid membrane skeletal protein in normal and diseased human skin." Histology and Histopathology. in press.

    • Related Report
      1995 Annual Research Report
  • [Publications] Shimizu T: "Immunoreactive analogues of erythrocyte ankyrin in human epidermal keratinocyte" Arch Dermatol Res. in press.

    • Related Report
      1995 Annual Research Report
  • [Publications] Sato‐Matsumura KC: "Localization of annexin I(lipocortin I,P35)mRNA in normal and diseased human skin by in situ hydridization" Arch Dermatol Res. in press.

    • Related Report
      1995 Annual Research Report
  • [Publications] 小泉洋子: "神経ペプチドと皮膚アレルギー" 興和医報. 38. 644-646 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 小泉洋子: "皮膚の老化による表皮細胞膜情報伝達系の変化とその防止のための基礎的研究" 第4回資生堂皮膚老化研究ファンド研究報告書. 4. 2-5 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 小泉洋子: "ニューロペプチド" 皮膚科キーワード. 印刷中.

    • Related Report
      1995 Annual Research Report
  • [Publications] Koizumi H.: "Substance P induces inositol 1,4,5-trisphosphate and intracellular free calcium increase in cultured normal human epidermal keratinocytes" Experimental Dermatology. 3. 40-44 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Koizumi H.: "Differentiation associated localization of sprl in normal human skin and skin diseases" J Dermatol Sci. 8. 71 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Takano J.: "Ultraviolet action spectra for peroxide generation in human and pig epidermal keratinocytes loaded with dihydroxyrhodamine 123" Arch Dermatol Res. (in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Shimizu T.: "Localization of immunoanalogue of erythrocyte protein 4.1 and spectrin in epidermis of psoriasis vulgaris" Histochemistry. (in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Fujikawa K.: "Assignment of six patients with xeroderma pigmentosum in Hokkaido area to a variant form" J Radiat Res. 35. 168-178 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Koizumi H.: "Dermal dendrocyte hamartoma with stubby white hair:A novel connective tissue hamartoma of infancy" J Am Acad Dermatol. 32. 318-321 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 小泉洋子: "神経ペプチドと皮膚アレルギー" 興和医報. (印刷中).

    • Related Report
      1994 Annual Research Report
  • [Publications] Koizumi H.: "Differentiation associated expression of nPKC in normal human skin and skin diseases" Journal of Investigative Dermatology. 101. 858-863 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Koizumi H.: "Clinical significance of serum adenosine deaminase activity in patients with mycosis fungoides" Journal of Dermatology. 20. 394-399 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Koizumi H.: "Substance P induces inositol 1,4,5-trisphosphate and intracellular free calcium increase in cultured normal" human epidermal keratinocytes.Experimental Dermatology. 3(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Osada S.: "Predominant expression of nPKC,a Ca^<2+> independent isoform of protein kinase C in epithelial tissue" Cell Growth and Differentiation. 4. 167-175 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 小泉洋子: "ケラチノサイトの増殖調節 情報伝達系" 日本皮膚科学会雑誌. 103. 1614-1620 (1093)

    • Related Report
      1993 Annual Research Report
  • [Publications] 小泉洋子: "プロテインカイネースC" 皮膚科の臨床. 35. 1297-1302 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 小泉洋子: "ニューロペプタイド" 皮膚科の臨床. 35. 1325-1332 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 日野原重明: "今日の治療指針1993" 医学書院, 1304 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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