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Alopecia mechanism study of somousho chimeric mice

Research Project

Project/Area Number 05670723
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Dermatology
Research InstitutionTOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY

Principal Investigator

MOROHASHI Masaaki  Toyama medical and pharmaceutial University, Dermatology, Professor., 医学部・皮膚科, 教授 (50018719)

Co-Investigator(Kenkyū-buntansha) OHTSUYAMA Minoru  Same as above, instructor, 附属病院, 助手 (10213787)
KAGOURA Masayori  Same as above, instructor, 医学部, 助手 (50201413)
MATSUI Chihiro  Same as above, instructor, 附属病院, 助手 (10181679)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1994: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1993: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsMinoxidil / Calcium ion / Chloride channel / hair growth / testosterone / Whole cell clamp / Cell volume analysis / Keratinocyte / 細胞容積解析法 / 疎毛症キメラマウス / 電子顕微鏡 / 角化異常 / 5αレダクターゼ / Clチャネル / protein kinase C
Research Abstract

On the basis of the research in 1993, we have studied the effects of hair growth promoter on eccrine sweat gland cells and human epidermal keratinocytes instead of hair follicle cells because of easy obtaining. (1) Whole cell patch clamp technique showed that minoxidil sulfate, an active metabolite of minoxidil, opens primarily Cl-channels of eccrine clear cells and minoxidil opens their K-channels. Therefore hair growing is associated with not only vasodilatory and increasing blood flow due to K-channel opening but also Cl-channel opening. Cell volume analysis experiment also showed K-channel opening of keratinocytes pretreated with valinomycine. The effect of Cl-channel opening on hair growth still remains unknown. (2) We found that minoxidil sulfate decreases internal Ca^<2+> of keratinocytes and that testosterone increases their internal Ca^<2+>. This difference indicates that internal calcium concentration may be associated with hair growth. The further study using hair follicle cell are supposed to be continued.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Minoru OHTSUYAMA: "Stimulation of Cl Conductonce by Minoxidil Sulfate and K Conductance by Minoxidil in Eccrine Clear Cells" The Journal of Pharmacology and Experimental Therapeutics. 269. 823-831 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 大津山 實: "表皮角化細胞のClチャネルに及ぼすMXDS(Minoxidil suefate)の影響" 日本皮膚科学会雑誌. 104. 483- (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Minoru OHTSUYAMA: "Cell Violume Regulation in Hypotonic Medicum in Keratinocytes" Journal of Dermatological Science. 8. 69- (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.OHTSUYAMA,et al.: "Stimulation of Cl Conductance by Minoxidil Sulfate and K Conductorce by Minoxidil in Eccrine Clear Cells" The Journal of Pharmacology and Experimental Therapeatics. Vol.269. 823-831 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.OHTSUYAMA et al.: "The effect of Minoxidil sulfate on Cl-channels in Keratinocytes" The Japanese Journal of Dermatology. Vol.104. 483 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.OHTSUYAMA et al.: "Cell Volume Regulation in Hypotonic Medium in Keratinocytes" Journal of Dermatolog'cal Science. Vol.8. 69 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.OHTSUYAMA: "Stimulation of Cl Conductance by Minoxidil Sulfate and K Conductonce by Minoxidil in Eccrine Clean Cells" The Journal of Pharmacology and Experimental Therapeutics. 269. 823-831 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 大津山 實: "表皮角化細胞のclチャネルに及ぼすMXDS(Minoxidil sulefate)の影響" 日本皮膚科学会雑誌. 104. 483 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.OHTSUYAMA: "CELL VOIUME REGULATION IN HYPOTONIC MEDIUM IN KERATINOCYTES" Journal of Dermatological Science. 8. 69 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ohtsuyama M.et al: "Study of chloride channel regulation in autameous cell using cell volume image analysis of a single dissociated cell" Journal of Dermatological Science. 6. 116 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kagoura M.et al: "Genetic and histological analysis of samousho-mice" Journal of Dermatological Science. 2. 247 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] 籠浦正順 他: "疎毛症マウスの遺伝的、組織学的検討・キメラマウスの作成を含めて" バイオサイエンス研究会誌. 5. 8-18 (1992)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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