| Project/Area Number |
05670741
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Dermatology
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| Research Institution | Yokohama City University |
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Principal Investigator |
ISHII Norihisa YOKOHAMA CITY UNIVERSITY,SCHOOL OF MEDICINE,ASSISTANT PROFESSOR, 医学部, 講師 (50159670)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Shunichi YOKOHAMA CITY UNIVERSITY,SCHOOL OF MEDICINE,ASSISTANT, 医学部, 助手 (40236592)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1993: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Delayd-type hypersensitivity / 2,4-Dinitro-1-fluorobenzene (DNFB) / Early-acting reaction / Ear swelling / Footpad swelling / Mast cells / Nickel sulfate (NiSO_4) |
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| Research Abstract |
The elicitation in immunized mice of delayd-type hypersensitivity (DTH) responses to DNFB or NiSO_4 was found to be mediated by the sequential activities of two different antigen-specific Thy-1^+cells. Early-acting DNFB/NiSO_4-specific, DTH-initiating cells were required for elicitation of subsequent 24hr DNFB/NiSO_4-specific DTH and had an unusual phenotype for an antigen-specific cell (Thy-1^+, CD3^-4^-5^+8^-23^+, B220^+). In contrast, the late-acting, DNFB/NiSO_4-specific DTH-effector T cells were Thy-1^+, CD3^+4^+5^+8^-23^-, and B220^-. Our results led us to surmise that the early-acting DTH-initiating cells were necessary to locally recruit the late-acting effector T cells. We found that mast cells are important for expression of early-acting, DTH-initiating cell activity in this DNFB/NiSO_4-specific, DTH system. This is probably due to the microenvironmental absence of mast cells in mast cell-deficient WBB6F_1-W/W^Vmice.
Our results indicate that two different antigen-specific Thy-1^+ cells are necessaty to elicit DNFB/NiSO_4-specific DTH in mice and that mast cells are necessaty for expression of the early component that is due to early-acting, DTH-initiating cells.
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