Interaction of type I and type IV reaction in allergic diseases-fundamental research using mouse

Research Project

Project/Area Number	05670741
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	Dermatology
Research Institution	Yokohama City University
Principal Investigator	ISHII Norihisa YOKOHAMA CITY UNIVERSITY,SCHOOL OF MEDICINE,ASSISTANT PROFESSOR, 医学部, 講師 (50159670)
Co-Investigator(Kenkyū-buntansha)	TANAKA Shunichi YOKOHAMA CITY UNIVERSITY,SCHOOL OF MEDICINE,ASSISTANT, 医学部, 助手 (40236592)
Project Period (FY)	1993 – 1994
Project Status	Completed (Fiscal Year 1994)
Budget Amount *help	¥2,100,000 (Direct Cost: ¥2,100,000) Fiscal Year 1994: ¥800,000 (Direct Cost: ¥800,000) Fiscal Year 1993: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywords	Delayd-type hypersensitivity / 2,4-Dinitro-1-fluorobenzene (DNFB) / Early-acting reaction / Ear swelling / Footpad swelling / Mast cells / Nickel sulfate (NiSO_4)
Research Abstract	The elicitation in immunized mice of delayd-type hypersensitivity (DTH) responses to DNFB or NiSO_4 was found to be mediated by the sequential activities of two different antigen-specific Thy-1^+cells. Early-acting DNFB/NiSO_4-specific, DTH-initiating cells were required for elicitation of subsequent 24hr DNFB/NiSO_4-specific DTH and had an unusual phenotype for an antigen-specific cell (Thy-1^+, CD3^-4^-5^+8^-23^+, B220^+). In contrast, the late-acting, DNFB/NiSO_4-specific DTH-effector T cells were Thy-1^+, CD3^+4^+5^+8^-23^-, and B220^-. Our results led us to surmise that the early-acting DTH-initiating cells were necessary to locally recruit the late-acting effector T cells. We found that mast cells are important for expression of early-acting, DTH-initiating cell activity in this DNFB/NiSO_4-specific, DTH system. This is probably due to the microenvironmental absence of mast cells in mast cell-deficient WBB6F_1-W/W^Vmice. Our results indicate that two different antigen-specific Thy-1^+ cells are necessaty to elicit DNFB/NiSO_4-specific DTH in mice and that mast cells are necessaty for expression of the early component that is due to early-acting, DTH-initiating cells.