Pharmacokinetic Study of Targeting Tumors with Radio-and Drug-conjugated Monoclonal Antibody
| Project/Area Number |
05670785
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Radiation science
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| Research Institution | Keio University School of Medicine |
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Principal Investigator |
NAKAMURA Kayoko Keio Univ.School of Med.Dept.of Radiol, Assist.Prof., 医学部, 専任講師 (20124480)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1993: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Radiolabeled Antibody / Interferon / Interleukin-2 / Targeting / Tumor / De-ionization / 癌 |
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| Research Abstract |
Using athymic mice bearing human tumor xenografts, the effects of several drugs on the pharmacokinetics and biodistribution of radiolabeled monoclonal antibody were investigated. The following results were obtained :
(1)The administration of Interferon-minipellet which consisted of collagen and natural interferon-alpha could keep the blood interferon level constant for 5 days. It also enhanced the uptake of radiolabeled anti-CEA monoclonal antibody in tumors with twice comparing with control mice. It increased in the clearance of In-111 from blood, thus making the dosimetry of Y-99m-labeled antibody favorable for radioimmunotherapy.
(2)The administration of IL-2 with low dose increased the uptake of Tc-99m-labeled antibody not in the tumor but also in some normal tissues. IL-2 could be conjugated with antibody without loss of its action which increases in the permeability of blood vessels. The biodistribution study of radiolabeled antibody conjugated with IL-2 is currently underway.
(3)The administration of verapamil, lidocaine, monensin, or chloroquine could make tumor retention time of I-125-labeled antibody longer. They increased the blood clearance rate of I-125, thus increasing the tumor-to-blood ratio remarkably.
These experimental results indicated that drugs investigated augment the efficacy of targeting of tumors with iodine-labeled antibody.
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Report
(3 results)
Research Products
(33 results)
