| Project/Area Number |
05670794
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Radiation science
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| Research Institution | National Institute of Health Japan |
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Principal Investigator |
GOTOH Eisuke National Institute of Health Japan, Chief Researcher, 遺伝子資源室, 主任研究官 (90215484)
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| Co-Investigator(Kenkyū-buntansha) |
関根 広 東京慈恵会医科大学, 放射線科, 助手 (40187852)
亀岡 洋祐 国立予防衛生研究所, 遺伝子資源室, 厚生技官 (00224692)
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| Project Period (FY) |
1993 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1993: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | Local irradiation therapy / Late effect / Chromosomal aberration / in situ hybridization / Chromosome painting / PCC / Okadaic acid / Gold in situ hybridization / 高線量照射 / カリクリンA / 金インサイツ雑種形成法 |
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| Research Abstract |
To ascertain the irradiation dose for whole body and estimate the subsequent late effect after local irradiation therapy, we performed the fluorescent in situ hybridization (FISH) using centromere specific probe and analyzed chromosomal aberration found in peripheral blood lymphocytes of patients.Patients subjected to local irradiation of 50 Gy of 10 MV X-ray for uterus cervical cancer or administration of 100-150 mCi of radioiodide for thyroid cancer seemed to be irradiated on their whole body 1.0 Gy or 0.7 Gy of 10 MV X-ray, respectively.
Chromosome painting study on the same samples revealed that the estimated irradiation dose was higher than that of obtained by the FISH tudy using centromere specific probe.Therefore, chromosome painting will be more sensitive method for detecting chromosomal aberration generated by irradiation.
Increasing the dose of irradition generate higher damaged chromosomal aberration.However, the higher the dose, the higher the domages of cells, resulting in arresting of the cell cycle.Therefore it is difficult, or even impossible toobtain metaphase chromosomes.
Premature chromosome condensation can produce chromosomes from the highly damged cells by irradiation.In fact, okadaic acid or calyculin A could effectively produce metaphase like chromosomes in damaged cells subjected 40 Gy irradiation.This method allows to estimate the dose response in high range irradiation, which has not been able to do so far.This method also allows to generate chromosomes from the cells which viability is too low to obtain metaphase chromosomes using colcemid.
I also developed the in situ hybridization method using immunogold silver staining method.This method allows to observe specimens in light illumination and preserve it permanently.
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