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Identification of Abnormal expression of proteins in psychotomimetic-treated animals using molecular biological methods

Research Project

Project/Area Number 05670810
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Psychiatric science
Research InstitutionKobe University School of Medicine

Principal Investigator

SHIRAKAWA Osamu  Kobe University School of Medicine, Department of Psychiatry, Assistant Professor, 医学部・附属病院, 講師 (40243307)

Co-Investigator(Kenkyū-buntansha) NISHINO Naoki  Kobe University School of Medicine, Department of Psychiatry, Assistant Professo, 医学部, 講師 (30218177)
北村 登  神戸大学, 医学部・附属病院, 医員
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1994: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1993: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsschizophrenia / phencyclidine / methanphetamine / glutamate / transporter / 動物モデル
Research Abstract

1. To study the roles of Ca^<2+>-binding proteins in the pathology of schizophrenia, we determined the acute (1h) and delayd (24h) effects of PCP (8mg/kg, s.c.) on the gene expression of NVP (neural visinin-like Ca^<2+>-binding protein) -1 in the rat brain. After 24 hours, the NVP-1 mRNA level in the nucleus accumbens showed a significant decrease of 42%.
2. To examine the possible involvement of glutamate in the pathology of schizophrenia, we studied in the discrete rat brain regions the effect of methamphetamine (MAP) and phencyclidine (PCP) treatments on GLT-1 immunoreactivities (GLT-1-IR) , one of the glutamate transporter proteins specifically expressed in the brain, by western blot analysis. Single dose of MAP (4 mg/kg, s.c.) produced no significant changes in GLT-1-IR either one hour or 24 hours after the injection. Repetitive injections of MAP (each dose of 4 mg/kg, s.c., 5 times/week for three weeks) to rats in which we confirmed development of behavioral reverse tolerance to MAP produced a significant increase in GLT-1-IR in the caudate-putamen by about 50% (p<0.05). Single dose of PCP (8mg/kg, s.c.) produced no changes in GLT-1-IR studied one hour after the injection. Whereas, 24 hr after the injection GLT-1-IR decreased significantly in the hippocampus by about 30% (p<0.005) , with no changes in immunoreactivities of glial fibrillary acidic protein (GFAP) , a cell marker for astroglia. The increased GLT-1-IR in the caudate-putamen of rats which developed reverse tolerance to MAP suggests the relation of brain region-specific changes in glutamatergic neurotransmission to vulnerability to relapse in schizophrenic patients. The delayd response of GLT-1-IR in the hippocampus to PCP treatment may well explain an involvement of hippocampal glutamatergic abnormalities in the pathophysiology ofschizophrenia as well as the time latency in the development of human PCP psychosis.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Gao, X. M. et al.: "Delayed regional metabolic actions of phencyclidine." European J. of Pharmacol.241. 7-15 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 梶本康雄、ほか: "ラット脳における神経特異的ビジニン類似カルシウム結合蛋白質(NVP)mRNAの発現に対するフェンサイクリジンの急性及び遅延性の効果" 神経化学. 33. 384-385 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kajimoto, Y. et al.: "Delayed changes in neural visinin-like calcium-binding protein gene expression caused by acute phencyclidine administration" Journal of Neural Transmission. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Gao, X.M.et al.: "Delayd regional metabolic actions of phencyclidine." European J.of Pharmacol.241. 7-15 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kajimoto, Y.et al.: "Acute and delayd effects of phencyclidine in neural visinin-like calcium-binding protein mRNA in rat brain" Shinkeikagaku. 33,1. 384-385 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kajimoto, Y.et al.: "Delayd changes in neural visinin-like calcium-binding protein gene expression caused by acute phencyclidine administration" J.of Neural Transmission. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 梶本康雄、ほか: "ラット脳における神経特異的ビジニン類似カルシウム結合蛋白質(NVP)mRNAの発現に対するフェンサイクリジンの急性及び遅延性の効果" 神経化学. 33. 384-385 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kajimoto,Y.et al.: "Delayed changes in neural visinin-like calcium-binding protein gene expression caused by acute phencyclidine administration" Journal of Neural Transmission. (in press).

    • Related Report
      1994 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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