| Project/Area Number |
05670815
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Psychiatric science
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| Research Institution | OITA MEDICAL UNIVERSITY |
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Principal Investigator |
AKIYOSHI Jotaro OITA MEDICAL UNIVERSITY,DEPT OF NEUROPSYCHIATRY,ASSISTANT PROFESSOR, 医学部, 講師 (00159344)
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| Co-Investigator(Kenkyū-buntansha) |
TSUCHIYAMA Kounosuke OIAT MEDICAL UNIVERSITY,DEPT OF NEUROPSYCHIATRY,ASSISTANT SCIENTIST, 医学部, 助手 (80197730)
YAMADA Kenji OITA MEDICAL UNIVERSITY,DEPT OF NEUROPSYCHIATRY,ASSISTANT SCIENTIST, 医学部, 助手 (30210446)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1993: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | SEROTONIN / ANTIDEPRESSANT / CALCIUM / DEPRESSION / アニタゴニスト / アンタゴニスト / カルシウム / 脱感作 |
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| Research Abstract |
Serotonin 5-HT_<1C> receptor-mediated intracellular Ca^<2+> mobilization was investigated in 5-HT_<1C> receptors expressed in chinese hamster ovary (CHO) cells ; and fura-2/AM was used to investigate the regulation of 5-HT_<1C> receptor function.CHO cells, transfected with a cDNA clone for the 5-HT_<1C> receptor.The addition of 5HT mobilized intracellular Ca^<2+> in a dose-dependent fashion.Exposure of 5HT,a 5-HT receptor agonist, MCPP (1-(3-chlorophenyl)piperazine dihydrochloride), a 5-HT _<1C> agonist and DOI (1-(4-iodo-2,5-dimethoxyphenyl)-2-aminopropane), a 5-HT_<1C> and 5-HT_2 agonist, resulted in increased intracellular Ca^<2+> levels.
Mianserin, mesulergine, ritanserin and ketanserin each blocked 5-HT-mediated intracellular Ca^<2+> mobilization more effectively than spiperone.Mianserin and amoxapine inhibited 5-HT-mediated intracellular Ca^<2+> mobilization completely, amitryptyline, nortriptyline and imipramine reduced it about 50%.These results suggest that antagonism in CHO cells transfected with the human 5-HT_<1C> receptors is antiserortonergic effects of antidepressants.
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