| Project/Area Number |
05670841
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
内分泌・代謝学
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| Research Institution | Yamagata University (1994)
Chiba University (1993) |
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Principal Investigator |
SAITO Yasushi Yamagata univ.dept.of lab.medicine.professor, 医学部, 教授 (50101358)
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| Co-Investigator(Kenkyū-buntansha) |
村野 俊一 千葉大学, 医学部, 助手 (50231634)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1993: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | LDL-receptor / LDL / Binding domain / EGF / transmembrance / 動脈硬化 / 脂質代謝 / クローニング |
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| Research Abstract |
The cDNA clones which is similar to the LDL-receptor were selected by hybridization.
The probes were used as follows. One is the LDL-receptor binding domain and the other has no homology to the LDL-receptor. Using these two probes, we selected several clones and finally, we selected the clone which is homologous to the LDL-receptor but not LDL-receptor (LDL-receptor homologous protein LRHP).
The DNA sequence was determined. It has 6962 pb.Amino acid sequence has the EGF precursor domain, LDL binding domain and tramsmembrane domain. The amino acid sequence similarity to the known protein not detected. The function and its significance are now in progress.
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