| Project/Area Number |
05670867
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
内分泌・代謝学
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| Research Institution | The University of Tokushima |
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Principal Investigator |
KAWAI Hisaomi The University of Tokushima, School of Medicine, Associate Prof., 医学部, 助教授 (00035461)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIDA Yoshihiko The University of Tokushima, University Hospital of School of Medicine.Assistant, 医学部・付属病院, 講師 (30198478)
AKAIKE Masashi The University of Tokushima, University Hospital of School of Medicine.Assistant, 医学部・付属病院, 助手
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1993: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Hashimoto's thyroiditis / Basedow's disease / HTLV-I / HTLV-II / anti-HTLV-I antibody / HTLV-I infected rabbit / HTLV-I carrier / HTLV-II provirus / バセドー病 / 抗HTLV-I |
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| Research Abstract |
The anti-HTLV-I antibodies in the serum were studies by particle agglutination method and western blotting, and detected in 9 (6.3%) out of 144 patients with Hashimoto's thyroiditis and 5 (5.4%) out of 92 patients with Basedow's disease. These frequencies were significantly higher than the percentage (2.0%) among the general population in the same geographic area (p<0.01,0.05, respectively). In the group of bolld donors who tested postive for antibodies including anti-thyro-globulin antibody and antimicrosomal antibody. This percentage was higher than that (6.3%) in the group of blood donors who were negative for anti-HTLV-I antibody.
The presince of HTLV-II proviral DNA in the peripheral blood leukocytes was studied by PCR,and was found in 17 (51.5%) out of 33 patients with Hashimoto's thyroiditis, and 2 (11.7%) out of 17 patients with Basedow's disease. These frequencies were significantly (p<0.01) higher than that in the disease controls (1.9%). However, anti-HTLV-II antibodies were not detected in the serum. The base sequences of the PCR products were identical to that of control HTLV-II proviral DNA.Among the group of blood donors harboring anti-thyroid antibodies, HTLV-II proviral DNA was detected at high frequencies in individuals with or without anti-HTLV-I antibodies (26.0%, 14.3%, respectively).
In one of the two biopsy specimens of the thyroid from patient with Hashimoto's thyroiditis, HTLV-I protein and mRNA were detected by immunocytochemistry and in sity hybridzation, respectively.
Whole blood specimens from HTLV-I carriers were injected (i.v.) into rabbits to generate animal models. The thyroid functions and anti-thyroid antibodies were examined serially (work in progress).
These findings support the putative role of HTLV-I and HTLV-II viruses in the pathogenesis of a subset of patients with autoimmune thyroiditis.
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