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EXPRESSION OF MYELOID PHENOTYPES DURING HUMAN B-CELL DIFFERENTIATION : ANALYSIS OF CHRONIC LYMPHOCYTIC LEUKEMIA

Research Project

Project/Area Number 05670916
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Hematology
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

OKAMURA Seiichi  KYUSYU UNIVERSITY,THE FIRST DEPARTMENT OF INTERNAL MEDICINE,FACULTY OF MEDICINE,ASSISTANT PROFESSOR, 医学部, 講師 (20136435)

Co-Investigator(Kenkyū-buntansha) OHHNO Yujyu  KYUSYU UNIVERSITY,THE FIRST DEPARTMENT OF INTERNAL MEDICINE,FACULTY OF MEDICINE,, 医学部, 医員
IWASAKI Hiromi  KYUSYU UNIVERSITY,THE FIRST DEPARTMENT OF INTERNAL MEDICINE,FACULTY OF MEDICINE,, 医学部, 医員
KUBOTA Akira  KYUSYU UNIVERSITY,THE FIRST DEPARTMENT OF INTERNAL MEDICINE,FACULTY OF MEDICINE,, 医学部, 医員
IKEMATU Wataru  KYUSYU UNIVERSITY,THE FIRST DEPARTMENT OF INTERNAL MEDICINE,FACULTY OF MEDICINE,, 医学部, 医員
岩崎 浩巳  九州大学, 医学部, 医員
原田 直樹  九州大学, 医学部, 医員
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1993: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsCLL / DIFFERENTIATION ANTIGEN / IMMUNOGLOBULIN HEAVY CHAIN GENE / CD5 ANTIGEN / CD13 ANTIGEN / CD11b ANTIGEN / CD45RA ANTIGEN / VH4 GENE / 慢性B細胞性白血病 / 慢性リンパ性白血病 / CLL / フローサイトメトリー / 免疫グロブリン遺伝子 / CD5 / 骨髄系抗原 / B細胞 / モノクローナル抗体
Research Abstract

We investigated the surface expression of leukocyte differentiation antigens and the immunoglobulin (Ig) heavy chain variable region (VH) gene family utilization in leukemic cells from 26 Japanese patients with chronic B cell leukemias with special reference to CD5 antigen expression. CD5 was expressed on leukemic cells in 21 of 26 cases (CD5^+), but not in five cases (CD5^-). Myelomonocytic marker, CD13 antigen was expressed on the leukemic cells in all five CD5^- cases, but in none of CD5^+ cases Leukemic cells in CD5^- cases also expressed CD11b antigen more frequently than those in CD5^+ cases (80% vs 11%, p<0.01). Another myeloid marker, CD33 was expressed neither on CD5^+ nor CD5^- leukemic cells. CD22, a restricted B cell marker, was expressed more frequently on CD5^- leukemic cells than CD5^+ leukemic cells (80% vs 33%, p<0.05). Another restricted B cell activation marker, CD23 was expressed at similar frequency in both the CD5^+ and CD5^- groups (67% vs 60%). Although CD45RA was expressed on the majority of leukemic B cells, the CD45RA expression level was significantly higher among CD5^- cases than CD5^+ cases (p<0.01).
In the analysis of VH gene expressed in chronic B cell leukemias by PCR amplification, CD5^+ cases preferentially utilized VH4 family members (48%, 10/21). CD5^- cases, on the other hand, mainly utilized VH3 family (80%, 4/5).
Thus, from our present observation of an albeit limited patient population, we have found an association between VH gene family utilization and CD5 antigen expression in chronic B cell leukemias. We have also demonstrated the differential expression of myelomonocytic markers in the CD5^+ and Cd5^- chronic B cell leukemias. These result are in agreement with previous suggestions that CD5 positivity is the hallmark for distinct clinical entity commonly referred in the literature as chronic lymphocytic leukemia.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Ikematsu Wataru: "Surface phenotype and Ig heavy chain gene usage in chronic B-cell leukemia:Expression of myelomonocytic surface markers in CD5 chronic B-cell leukemia." Blood. 83. 2602-2610 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ikematsu Wataru: "Surface Phenotype and Immunoglobulin Heavy Chain Gene Usage in Chronic B Cell Leukemias." New York Academy of Science. (印刷中). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimoda Kazuya: "Granulocyte colony-stinulating factor and platelet aggregation." Lancet. 341. 633- (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimoda Kazuya: "Identification of a functional receptor for granulocyte colony-stimulating factor on platelets." Journal of Clinical Investigation. 91. 1310-1313 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kubota Akira: "The c-kit molecule and the surface immunophenotype of human acute leukemia." Lymphoma and Leukemia. 14. 421-428 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimoda Kazuya: "G-CSF induces tyrosine phosphorylation of the Jak2 protein in the human myeloid G-CSF responsive and proliferative cells,but not in mature neutrophils." Biochemica Biophysica Research Communication. 203. 925-928 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ikematsu W: "Surface phenolype and Ig heavy chain gene usage usage in chronic B-cell leukemia : Expression of myelomonocytic surface markers in CD5 chronic B-cell leukemia." Blood. 83. 2602-2610 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ikematsu w: "Surface phenotype and immunoglobulin heavy chain gene usage in chronic B cell leukemias." New York Academy of Science. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimoda K: "Granulocyte colony-stimulating factor and platelet aggregation." Lancet. 341. 633 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimoda K: "Identification of a functional receptor for granulocyte colony-stimulating factor on platelets." Journal of Clinical Investigation. 91. 1310-1313 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kubota A: "The c-kit molecule and the surface immunophenotype of human acute leukemia" Lymphoma and Leukemia. 4. 421-428 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimoda K: "G-CSF induces tyrosine phosphorylation of the Jak2 protein in the human myeloid G-CSF responsive and proliferative cells, but not in mature neutrophils." Biochemica Biophysica Research Communication. 203. 925-928 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] IIkematsu Wataru: "Surface phenotype and Ig heavy chain gene usage in chronic B-cell leukemia:ExDression of myelomonocytic surface markers in CD5 chronic B-cell leukemia." Blood. 83. 2602-2610 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ikematsu Wataru: "Surface phenotype and Immunoglobulin Heavy Chain Gene Usagein Chronic B Cell Leukemias." New York Academy of Science. (印刷中). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kubota Akira: "The c-kit molecule and the surface immunophenotype of human acute leukemia." Lymphoma and Leukemia. 14. 421-428 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Shimoda Kazuya: "G-CSF induces tyrosine phosphorylation of the Jak2 protein in the human myeloid G-CSF responsive and proliferative cells,but not in mature neutrolhils." Biochemica Biophysica Research Communication. 203. 925-928 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Shimoda K.: "Identification of a functional receptor for granulocyte colony-stimulating factor on platelets." Journal of Clinical Investigation. 91. 1310-1313 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Shimoda K.: "Granulocyte colony-stimulating factor and platelet agregation." Lancet. 341. 633- (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Shimoda K.: "Human macrophage colony-stimulating factor levels in cerebrospinal fluid." Cytokine. 5. 250-254 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ikematsu W.: "Phenotypic and immunoglobulin heavy chain gene analysis of B cell chronic lymphocytic leukemia(B-CLL):Expression of myelomonocytic markers in CD5 negative B-CLL." Blood. 印刷中. (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kuboota A.: "Augmented production of interleukin 6 by co-culture of human bone marrow adherent cells and human leukemic cells." Biomedicine and Pharamacotherapy. 47. 379-384 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kubota A.: "The c-kit molecule and the surface immunophenotype of human acute leukemia." Lymphoma Leukemia. (印刷中). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Tajiri J.: "Granulocyte colony-stimulating factor treatment of antithyroid drug-induced granulocytopenia." Archives of Internal Medicine. 153. 509-514 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Takamatsu Y.: "Cytokine production by peripheral blood monocytes and T cells during haematopoietic recovery after intensive chemotherapy." British Journal of Haematology. 83. 21-27 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Tanaka T.: "Differentiation of rat myelomonocytic leukemia cell line (c-WRT-7)cells by rat preadipocyte cell line,REC A16 in vitro." Journal of Clinical and Experimental Oncology. 119. 335-341 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ohhara N.: "Levels of serum granulocyte colony-stimulating factor in patients with cerebrovascular diseases." Life Science. 53. 939-943 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hayashi S.: "Sequential expression of lymphokine genes during phytohemagglutinin-stimulated mitogenesis of normal human peripheral mononuclear cells." Biomedicine and Pharmacotherapy. 47. 155-160 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Fukuda T.: "Predominance of myeloid antigen in CD34 positive peripheral blood stem cells over those in bone marrow,after the administration of granuloyte colony-stimulating factor." European Journal of Haematology. (印刷中). (1994)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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