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Correlation between cerebral energy metabolism using ^<31>P-NMR and cerebral blood flow : Pathophisiological studies on hypoxic-ischemic encephalopathy in newborn piglets

Research Project

Project/Area Number 05670974
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Embryonic/Neonatal medicine
Research InstitutionKagawa Medical School

Principal Investigator

ONISHI Shoju  Kagawa Medical School Professor, 医学部, 教授 (40080014)

大西 鐘寿 (1994)  香川医科大学, 医学部, 教授

Co-Investigator(Kenkyū-buntansha) IMAI Tadashi  Kagawa Medical School Insutructor, 医学部, 助手 (60176477)
ISOBE Kenichi  Kagawa Medical School Assistant Professor, 医学部・附属病院, 講師 (00159815)
ITOH Susumu  Kagawa Medical School Assistant Professor, 医学部, 講師 (80145052)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1993: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywords^<31>P-nuclear magnetic resonanse spectroscopy / Cerebral blood flow / Cerebral energy metabolism / Colored microsphere / Hypoxic-ischemic encephalopathy / Hypoxic looding / Newborn piglet / ^<31>P-MRスペクトロスコピー / カラードマイクロスフェレ / 血液生化学的検査 / 水素クリアランス / 脳循環 / 脳エネルギー代謝障害 / laser-doppler-velocimeter
Research Abstract

For the pathophysiological study of hypoxic-ischemic encephalopathy, using the experimental condition of 15 min of graded hypoxia, we measured cerebral energy metabolism using phosphorus 31 nuclear magnetic resonanse spectroscopy (<@D131@>D1P-NMR) and cerebral blood flow (total CBF) with colored microspheres in seven newborn piglets within 24 hours after birth, which resemble human newborns in terms of cerebral growth. Total CBF before hypoxic insulte (control) was 50.2(]SY.+-。[)6.5 ml/min/100g. At 15 minutes after the start of hypoxic insulte (FiO<@D22@>D2=0.08), CBF increased to 90.1(]SY.+-。[)19.1ml/min/100g and became 1.8 times as much as the control. However, at 45 minutes after hypoxia (FiO<@D22@>D2=0.05), total CBF decreased to 42.2(]SY.+-。[)12.0 ml/min/100g and no significant difference was found compared to control. With regards to bradycardia (HR<100), total CBF was significantly decreased to 18.3(]SY.+-。[)9.4 ml/min/100g. On brain stem (pons, mid brain and medulla), blood flow was significantly increased at 45minutes, too, and was not significantly decreased on bradycardia. Compared to total CBF,considerably more blood flow was measured at 45 minutes and on bradycardia.
There was a significant correlation between mean arterial blood pressure (MABP) and CBF (r=0.861, P<0.001). The autoregulation of total CBF was proved to be disturved. The Phosphocreatinine (PCr) /inorganic phosphate (Pi) and intracellular pH (pHi) did no change until total CBF reached levels below the levels control. The PCr/Pi and pHi suddenly decreased when total CBF decreased below the control. There was a significant correlation between total CBF and the PCr/Pi or pHi (r=0.843, p<0.001 and r=0.796, P<0.001). There was no significant correlation between PaO_2 and the PCr/Pi.
Therefore, these results suggest that CBF is the most important factor in maintaining cerebral energy metabolism on hypoxic-ischemic encephalopathy.

Report

(2 results)
  • 1994 Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] 杉原聡: "^<31>P-NMRによる脳内エネルギー代謝と脳血流の関係-新生仔豚による低酸素性虚血性脳症の病態生理学的研究-" 日本未熟児新生児学会雑誌. 6. 97-105 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 今井正: "Magnetic resonance spectrometry" 周産期医学. 25. 879-883 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 今井正: "新生児中枢神経障害の診断と管理" 産婦人科治療. 68. 22-29 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 大西鐘壽: "序論パネルディスカッション「胎児・新生児中枢神経障害の診断、管理、予後判定」" 日本新生児学会雑誌. 30. 27-40 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 磯部健一: "周産期の臨床検査 新生児編 MRS(magnetic resonance spectroscopy)" 周産期医学. 23. 444-449 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 大西鐘壽: "脳のエネルギー代謝とその障害" 日本未熟児新生児学会雑誌. 5. 77-101 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Satoshi Sugihara, Takashi Kusaka, Tadashi Imai, Kenichi Isobe, Susumu Itoh and Shoju Onishi: "Correlation between cerebral energy metabolism and brain blood flow using ^<31>P-NMR -Pathophysiological studies on hypoxic-ischemic encephalopathy in newbornpiglets-" Journal of Japan Society for Premature and Newborn Medicine. 6. 97-105 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tadashi Imai: "Magnetic resonance spectrometry" Perinatal Medicine. 25. 879-883 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tadashi Imai, Kenichi Isobe and Shoju Onishi: "Diagnosis and management of neonatal cerebral damage" Obstetrical and Gynecological Therapy. 68. 22-29 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shoju Onishi: "Diagnosis, management and prognostic evalution on fetal and neonatal CNS disturbances" Acta Neonatologica Japonica. 30. 27-40 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kenichi Isobe, Masatoshi Kondo, Tadashi Imai, Susumu Itoh and Shoju Onishi: "Laboratory examination in the perinatal period magnetic resonance spectroscopy" Perinatal Medicine. 23. 444-449 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shoju Onishi, Kenichi Isobe, Susumu Itoh, Masatoshi Kondo, Tadashi Imai and Konomu Hirao: "Cerebral energy metabolism and its disturbances in the neonatal period" Journal of Japan Society for Premature and Newborn Medicine. 5 (1). 77-101 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 大西鐘壽: "脳のエネルギー代謝とその障害" 日本未熟児新生児学会雑誌. 5. 77-101 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 大西鐘壽: "周生期脳障害の診断と治療 序論" 日本新生児学会雑誌. 30. 195-207 (1994)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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