| Project/Area Number |
05670984
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Tohoku University |
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Principal Investigator |
SATOMI Susumu Tohoku University, Medicine., Professor, 医学部, 教授 (00154120)
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| Co-Investigator(Kenkyū-buntansha) |
KATAYAMA Masafumi Tohoku University, Medicine, Research Associate, 医学部附属病院, 助手 (00177411)
DOI Hideyuki Tohoku University, Medicine,, 医学部附属病院, 助手 (90188839)
KATO Hirotaka Tohoku University, Medicine, Research Associate, 医学部附属病院, 助手 (00240656)
SUGAMURA Kazuo Tohoku University, Medicine, Professor, 医学部, 教授 (20117360)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1993: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | IL-2 receptor / anti-IL-2Ralpha antibody / anti-IL-2Rbeta antibody / anti-IL-2Rgamma antibody / skin allograft / 抗γ鎖抗体 / MLC / IL-2の吸収 / 細胞内刺激伝達抑制 / 遊離IL-2受容体 / 皮膚移植延長効果 |
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| Research Abstract |
The IL-2 receptor (IL-2R) gamma chain, so-called common gamma (gammac) chain, which is shared with multiple cytokine receptors, plays important roles in the immune system. We here assessed the immunosuppressive ability of mAb specific for the gammac chain in induction of CTLs and allograft rejection in combination with mAbs specific for the alpha and beta chains of IL-2R.CBA/N (H-2^k) mice were injected intraperitoneally with allogeneic splenocytes from BALB/c (H-2^d) mice, and then administered with combinations of anti-IL-2Ralpha, anti-IL-2Rbeta and anti-gammac mAbs or a control mAb. Addition of anti-gammac mAb together with anti-IL-2Ralpha and anti-IL-2Rbeta mAbs induced a complete inhibition of CTL response. The numbers and populations of CD4^+CD8^- and CD4^-CD8^+ T cells were not significantly affected by administration of the three anti-IL-2R mAbs, whereas NK cells were completely depleted in spleens of mice treated with the anti-IL-2R mAbs. Furthermore, skin allograft survival was also significantly prolonged by administration of the three anti-IL-2R mAbs. These results suggest that the anti-gammac mAb in combination with anti-IL-2Ralpha and anti-IL-2Rbeta mAbs is capable of suppressing induction of CTLs and NK cells, resulting in prolongation of skin allograft survival.
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