| Project/Area Number |
05670988
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | University of Tsukuba |
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Principal Investigator |
ISHIKAWA Shigemi Inst. of Clin. Med., University of Tsukuba Assitant prof., 臨床医学系, 講師 (60232253)
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| Co-Investigator(Kenkyū-buntansha) |
ONIZUKA Masataka Inst. of Clin. Med., University of Tsukuba Assitant prof., 臨床医学系, 講師 (40214176)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1994: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1993: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | Endothelin / Lung lymph / Hypoxia / 肺循環 |
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| Research Abstract |
To investigate whether hypoxia causes the production of endothelin in lung, the content of endothelin (ET-1) was analyzed in the pulmonary circulation and lung lymph during and after hypoxia in sheep. The efferent duct of the caudal mediastinal node (CMN) was cannulated and the lung lymph was collected. The lung was ventilated with normoxic gas, and with 10%O_2 as hypoxia for 1 hr, then with normoxic gas for over 3hr. The ET-1 concentrations were measured in plasma and lymph using ELISA.The net output of ET-1 in the lung lymph was calculated as the lymph flow multiplied by the ET-1 concentration in lymph.
In the perfused lung, the ET-1 concentration in the perfusate was 3.3<plus-minus>2.0pg/ml before hypoxia, and was 5.7<plus-minus>2.5pg/ml during hypoxia. It was 9.2<plus-minus>4.5pg/ml 3 hr after the end of hypoxia(p<0.05 vs baseline). The net ET-1 output in lung lymph was 104.0<plus-minus>42.7pg/min in the baseline, and was 230.7<plus-minus>128.5pg/min. It was 395.2<plus-minus>175.8pg/min 1hr after the end of hypoxia (p<0.05 vs baseline) and kept significant higher level af
In the anesthetized sheep, the ET-1 concentration in the plasma was 1.1<plus-minus>0.1pg/ml before hypoxia, and was 1.6<plus-minus>0.5pg/ml during hypoxia (p<0.05 vs baseline). However it decreased to the baseline value 2 hr after the end of hypoxia. The net ET-1 output in lung lymph was 248.8<plus-minus>160.4pg/min in the baseline and 277.4<plus-minus>199.2pg/min during hypoxia. The change was not significant during and after
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