| Project/Area Number |
05670995
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
General surgery
|
|---|
| Research Institution | Niigata University |
|---|
Principal Investigator |
YOSHIKAWA Keiji Niigata University Hosptal, Emergency and Critical Care Unit, Associate Professor, 医学部・附属病院, 助教授 (60143765)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SATO Nobuaki Niigata University Hosptal, Surgery I.Lecturer, 医学部・附属病院, 講師 (90215822)
TAMIYA Youichi Niigata University Hosptal, Operation Center, Associate Professor, 医学部・附属病院, 助教授 (70163681)
|
|---|
| Project Period (FY) |
1993 – 1995
|
| Project Status |
Completed (Fiscal Year 1995)
|
| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1993: ¥900,000 (Direct Cost: ¥900,000)
|
|---|
| Keywords | [1-^<14>C]-leucine / protein kinetics / constant infusion / severe sepsis / tatal parenteral nutrition / 〔1-^<14>C〕-ロイシン / 〔1-^^<14>C〕-ロイシン |
|---|
| Research Abstract |
Procedures of protein kinetics analysis by the[1-^<14>C]-leucine constant infusion method was established using male Wistar rats weighing 450-500g. Particularly, establishing the step of collecting expired CO_2 (involving ^<14>CO_2) was a difficult point.
Dose of 250nCi/100g body weight/hr of[1-^<14>C]-leucine was sufficient enough to obtain the plasma ^<14>C-leucine specific activity and the ^<14>CO_2 radioactivity. Equilibration time of 4 hours was also confirmed.
Data obtained in the present study is as follows (mean values) ; plasmaleucine flux : 1334 mg/kg/day, leucineoxidation rate : 425mg/kg/day, rate ofleucine incorporation into protein : 909mg/kg/day, whole body protein synthesis rate : 11.6g/kg/day, amino acid oxidation rate : 5.5g/kg/day, whole body protein breakdown rate : 14.7g/kg/day.
This experimental procedures will be used to elucidate the protein and leucine metabolic kinetics in severe sepsis.
|
