| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1993: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Research Abstract |
We previously found that the contractile motility of the jejunum was increased 4 weeks after transplantation, and that dominant intrinsic neural component was changed from cholinergic to non-adrenergic, non-cholinergic (NANC). The present study investigated the long-term effects of transplantation on jejunal motility using rats that survived for 2 years postoperatively. Jejunal strips were harvested various group of rats, and intestinal motility was assessed by electrical transmural stimulation. Stimulation produced a similar increase of contraction at 4 weeks and 2 years after grafting. Pretreatment with atropine showed that the cholinergic component of contraction was respectively 45%, 24%, 32%, and 24% in adult controls, adult rats 4 weeks after transplantation, 2-year-old controls, and rats 2 years after transplantation. The NANC component (obtained with atropine and guanethidine) in each group was respectively 56%, 73%, 60%, and 69%. The actual valve of tetrodotoxin-insensitive myogenic component was significantly increased at 2 years after transplantation. These results suggested that the NANC component has a key role in the motility of transplanted small bowel throughout the life of the grafts.
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