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PATHOGENESIS OF PANCREATIC ACINAR CELL DAMAGE AND POSSIBILIT OF INHIBITION : STUDIES WITH EXPERIMENTAL PANCREATITIS IN RATS

Research Project

Project/Area Number 05671048
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Digestive surgery
Research InstitutionUniversity of Tokyo

Principal Investigator

KIMURA Wataru  University of Tokyo, Department of Intermal Medicine, Associate Profe, 医学部(病), 助手 (00169947)

Co-Investigator(Kenkyū-buntansha) 韓 一秀  東京大学, 医学部(病), 医員
INOUE Tomomi  University of Tokyo, Department of Intermal Medicine, Medical Fello, 医学部(病), 医員
FUTAKAWA Noriaki  University of Tokyo, Department of Intermal Medicine, Medical Fello, 医学部(病), 医員
SHINKAI Hiroshi  University of Tokyo, Department of Intermal Medicine, Medical Fello, 医学部(病), 医員
HAN Ilsoo  University of Tokyo, Department of Intermal Medicine, Medical Fello
井上 知己  東京大学, 医学部(病), 医員
大谷 泰一  東京大学, 医学部(病), 医員
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1994: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1993: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsUTI / Albumin / Image Analysis System / 通院解析装置 / 画像解析装置
Research Abstract

The pathogenesis of the acute pancreatitis still raises many questions. Trypsin is still thought to play an important role. Lipase, via its action on triglycerides, liberates free acids, which destroy intact cells. Another lipolytic enzyme phospholipase A2, via its action on lecithin, liberates lysolecithin, which also destroy intact cells. In the present study, we used the well characterized model of rat pancreatic acini to study possibilities to inhibit the acute cellular damage.
[Methods] : 1) Sodium-taurocholate was injected into the pancreatic duct of isolated rat pancreas and the organ continuously perfused with buffer +/- either urinary trypsin inhibitor (UTI) or albumin. Organ damage was evaluated by measurement of amylase and lipase in the portal effluence. 2) Either normal saline or 5% sodium-taurocholate was injected into the main pancreatic duct. At the same time, either HEPES-Ringered buffer or albumin, was injected into the interstitium of the whole pancreas. Six hours aft … More er the operation, rats were killed and spread of fat and parenchymal necrosis of the pancreas was grossly and histologically examined.
[Results] : Condition 1) Albumin was very effective and significantly lowered the release of enzymes into the portal venous effluence. UTI was ineffective. However, when we histologically examined using Olympus Image Analysis System, a high concentration of UTI significantly lowered both taurocholate-induced parenchymal necrosis and interstitial edema in the pancreas. Condition 2) Most of the pancreas was necrotic by the action of taurocholate in the control group, in which HEPES-Ringered buffer was injected into the interstitium of the pancreas. However, when albumin was injected, the necrosis of the pancreas by taurocholate injection of the pancreatic duct, was almost inhibited. These results were confirmed in the histological study.
[Conclusion] Albumin may have therapeutical implications due to its ability to bind detergents such as fatty acids and lysolecithin. UTI might inhibit necrosis and interstitial edema in acute pancreatitis. Less

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] 木村 理: "急性膵細胞壊死の発生機序-リゾレシチンと遊離脂肪酸" 胆と膵. 14. 849-852 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 木村 理 他: "重症急性膵炎-その病態と治療-" 日本腹部救急医学会雑誌. 13. 645-651 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 木村 理 他: "急性膵炎に対するドレナージ" 手術. 48. 2171-2179 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kimura W, et al.: "Effects of the trypsin inhibitor urinastain on taurocholate-induced pancreatic cellular damage in rats." Pathophysiology. (Submitted).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kimura W,Kuroda A,Muto T,Moessner J.: "Critical factors in the development of acute acinar cell necrosis-lysolecithin and free fatty acid." Biliary tract & Pancreas. 14. 849-852 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kimura W,Sata N,Kuroda A,Muto T,Kubota T,Imanari T,Terashima H.: "Severe acute pancreatitis-the pathogenesis and treatment-." J Abdominal Emergency Medicine. 13. 645-651 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kimura W,Maruyama Y,Sata N,Muto T.: "Abdominal drainage for severe acute pancreatitis." Operation. 48. 2171-2179 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kimura W,Furuhashi S,Zhang Q,Muto T.: "Effects of the trypsin inhibitor urinastatin on taurocholate-induced pancreatic cellular damage in rats." Pathophysiology. (submitted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 木村 理: "急性膵細胞の壊死の発生機序-リゾレシチンと遊離脂肪酸-" 胆と膵. 14. 849-852 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] 木村 理 他: "重症急性膵炎-その病態と治療-" 日本腹部救急医学会 雑誌. 13. 645-651 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] 木村 理 他: "急性膵炎に対するドレナージ" 手術. 48. 2171-2179 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kimura.W,et al: "Effects of the trypsin inhibitor urinastain on taurocholate-induced pancreatic cellular damage in rats." Pathophysiology. (submittecl).

    • Related Report
      1994 Annual Research Report
  • [Publications] 木村 理 他: "急性膵細胞壊死の発生機序-リゾレシチンと遊離脂肪酸-" 胆と膵. 14. 849-852 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 木村 理 他: "重症急性膵炎-その病態と治療-" 日本腹部救急医学会雑誌. 13. 645-651 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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