Effect of glucagon and hepatocyte growth factor on liver regeneration following resection of cirrhotic liver
| Project/Area Number |
05671089
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | The Jiki University, School of Medicine |
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Principal Investigator |
FUJITA Tetsuji The Jikei University, School of Medicine, Department of Surgery, Lecturer, 医学部, 講師 (60209062)
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| Co-Investigator(Kenkyū-buntansha) |
SAEKI Tomoyuki Assistant, 医学部, 助手 (50256385)
尾高 真 , 助手 (20233554)
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| Project Period (FY) |
1993 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1993: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | Liver Regeneration / Hepatocyte Growth Factor / Glucagon / グルタミン |
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| Research Abstract |
Although we have studied the effect of hapatocyte growth factor (HGF) on liver regeneration in the cirrhotic rat, a significant effect of HGF failed to be shown. It has been reported that glucagon functions as a co-mitogen of HGF.Taking it into consideration, we investigated the impact of HGF and/or glucagon on rat liver regeneration
1. Effect of HGF and/or glucagon on rat liver regeneration : We performed 70 % hepatectomy in the Wistar rats according to the method of Higgins and Anderson. Thereafter, these rats were divided into the 4 groups. Rats in group A were given neither glucagon nor HGF after hepatectomy. Those in group B were given 0.1 mg/kg of glucagon subcutaneously every 12 hours until 48 hours after hepatectomy. In group C,laparotomy was carried out again 6 hours after hepatectomy to administer 4 mug/kg of HGF into the portal vein. Animals in group D received both glucagon and HGF injections. A total of DNA content in the remnant liver and a ratio of proliferating cell nuclear antigen (PCNA)-positive cells were meassured. DNA content of the remnant liver (48 hours after hapatectomy) in group B was statistically higher than that in group A (12.04<plus-minus>3.25 (mean<plus-minus>SD) mg vs 8.26<plus-minus>1.68 mg, p<0.05). There was no difference in PCNA-positive cells between the two groups. There was no difference in DNA content between the group A and the group C,nor between the group B and the gr
2. Effect of glucagon on liver regeneration in rats with abnormal glucose metabolism : Effect of glucagon administration was investigated in the rats whose beta-cells had been destroyed by intravenous injection of streptozotocin (60mg/kg). Glucagon administration increased DNA content in the remnant liver (from 6.33<plus-minus>0.94mg to 10.11<plus-minus>1.65mg, p<0.
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Report
(4 results)
Research Products
(2 results)
