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Photodynamic effects using a diode laser with mono-L-aspartyl Chlorin e6 for implanted mice tumor

Research Project

Project/Area Number 05671135
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Thoracic surgery
Research InstitutionTokyo Medical College

Principal Investigator

OKUNAKA Tetsuya  Dep of Surgery Tokyo Medical College Iectuver, 医学部, 助手 (80233469)

Co-Investigator(Kenkyū-buntansha) TSUCHIDA Takaaki  Dep of Surgery Tokyo Medical College Iectuver, 医学部, 助手 (80256239)
FURUKAWA Kinya  Dep of Surgery Tokyo Medical College Iectuver, 医学部, 助手 (20246292)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1994: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1993: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsPhotodynamic Therapy (PDT) / Diode Laser / Mono-L-aspartyl Chlorin e6 (NPe6) / Photobleaching / Photodynamic Thevopy(PDT) / Mono-L-aspartyl Chlorin e6(NPe6) / 光線力学的治療(PDT) / NPe6 / ダイオードレーザー / 腫瘍親和性光感受性物質
Research Abstract

Photodynamic therapy (PDT) using the photosensitizer drug Photofrin has recently been approved for the treatment of early stage lung cancer, esophageal cancer, gastric cancer, uterine cancer and uterine dysplasia by the Japanese Ministry of Health and Welfare. The authors performed photodynamic therapy, avording any hyperthermic effects, using a diode laser and another photosensitizer mono-L-aspartyl chlorin e6 (NPe6) in an implanted fibrosarcoma in mice and achieved tumor therapeutic effects. The tissue temperature rise was 3.8゚C when 100mW/cm^2 light was delivered, this temperature being below the temperature range of hyperthermia. In the present study, PDT was performed 5 hours following the administration of 5.0 mg/kg NPe6. With 50,100,150 and 200 J/cm^2, tumor cure rates were 20,50,70 and 90%, respectively. In addition, when PDT was performed with administration of 1.25,2.5,5.0,7.5 and 10.0 mg/kg NPe6 with 100 J/cm^2 laser exposure, tumor cure rates were 0,20,50,70 and 90%, respectively. In some arimals, tumor recurrence seemed to occur from deeper layr of tumors. Therefore, the relationship between the effects of PDT and residual amounts of NPe6 in the deeper level of tumors following the PDT procedure were examined. We used a CCD camera system to measure the fluorescence intensity to evaluate the residual amount of NPe6 at 3mm from the tumor surface. The ratio of the residual amount of NPe6 following 50,100,150 and 200J/cm^2 laser exposure to no laser exposure were 73.4,36.0,22.0 and 15.9% respectively. These results suggest that a certain increase in the tumor tissue level of NPe6 and a certain increase of laser light dose reaching deeper layr of tumor caused an increase in percent cure. In addition, the effectiveness of PDT and also tumor recurrence following PDT depend to some extent on the total laser laser dose reaching deeper layrs of tumors. Furthermore, the effectiveness of PDT tends to correlate with the amount of photobleaching by PDT.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] 勝海東一郎: "Photodynamic Therapy Using a Diode Laser with NPe6 for Implanted Fibrosarcoma in Mice" Japanese Journal of Cancer Research. 85. 1165-1170 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 勝海東一郎: "Photodynamic Effect Using a Diode Laser with Mono-L-aspartyl Chlorin e^6 for Implanted Mouse Tumor" Journal of Clinical Laser Medicine & Surgcry. 12. 211-213 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 勝海東一郎: "半導体レーザーとNPe6を用いた光線力学的治療の検討" 第14回日本レーザー医学会 大会論文集. 14. 347-350 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 奥仲哲弥: "呼吸器系癌に対するPDT(喉頭癌、肺癌)" 日本レーザー医学会誌. 5. 21-28 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 奥仲哲弥: "Present Stotus of Endoscopic Treatment of Lung Cancer" 気管支学.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 加藤治文: "Ten year Disease-Free Survival of Lung Cancer Patient Tyeated Only by PDT" Laser in the Life Science.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Toichiro, Katsumi: "Photodynamic Theropy Using a Diode Laser with NPe6 for Implanted Fibrosarcome in Mice." Japanese Journal of Cancer Research. Vol.85-No11. 1165-1170 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Toichiro, Katsumi: "Photodynamic Effect Using a Diode Laser with Mono-L-aspartyl chlorin e6 for Implanted Mouse Tumor." Jourral of Claical Laser Medicine & Surgery. Vol.12-No4. 211-213 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Toichiro, Katsumi: "Photodynamic Theropy Using a Diode Laser with Mono-L-aspartyl Chlovin e6." Proc 14th Cangr.Jpn.Soc.Laser Med.Vol.14. 347-350 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tetsuya, Okunaka: "Photodynamic Theropy for Respiratory Cancer." Japanese Jourral of Laser Med.Vol.15. 21-28 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tetsuya, Okunaka: "Present Status of Endoscopic Treatment of Lung Cancer" Broachology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Harubumi, Kato: "Ten year Disease-Free Survival of Lung Cancer Patient Treated Only by PDT" Laser in the Life Science.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 勝海 東一郎: "Photodynamic Thevapy using a diode laser with Npe 6 for isnplanted fibrosarcoma in mice" Japanese Journal of Cancer Research. 85. 1165-1170 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 勝海 東一郎: "Photodynamic Effect Using a Diode Laser with Mono-L-aspartyl Chlorin e6 for Implanted Mouse Tumor." Journal of Ceinical Laser Medicine & Sargery. 12. 211-213 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 勝海 東一郎: "半導体レーザーとNPe6を用いた光線力学的治療の検討" 第14回.日本レーザー医学会 大会論文集. 14. 347-350 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 奥仲 哲弥: "呼吸器系癌に対するPDT(喉頭癌、肺癌)" 日本レーザー医学会誌. 5. 21-28 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 奥仲 哲弥: "Present Stotus of Endoscoptc Treatment of Ling Cancer" 気管支学. 16. (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 加藤 治文: "Ten year Disease-Fvee Survival of Lung Caner Patient Treatelo Only by PDT." Laser in the Life Scionco. 6. (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 勝海東一郎: "半導体レーザーとNPe6を用いた光線力学的治療(PDT)の検討" 日本レーザー医学会 第14回大会論文集. 14(予定). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 奥仲哲弥: "肺癌のレーザー療法" BIOTHERAPY. 7. 673-680 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 奥仲哲弥: "進行肺癌に対するレーザー治療の適応と限界" 気管支学. 15. 733-737 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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