| Project/Area Number |
05671149
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | UNIVERSITY OF TOKYO |
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Principal Investigator |
KIM Phyo (1994) Univ of Tokyo, Dept Neurosurgery, Assistant Professor, 医学部(病), 助手 (90231290)
根本 繁 (1993) 東京大学, 医学部(病), 助手 (20228290)
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| Co-Investigator(Kenkyū-buntansha) |
NONOMURAL Yoshiaki Univ of Tokyo, Dept Pharmacology, Professor., 医学部(医), 教授 (80009993)
SASAKI Tomio Univ of Tokyo, Dept Neurosurgery, Associate Prof., 医学部(病), 助教授 (10134561)
YOSHIMOTO Yuhei Univ of Tokyo, Dept Neurosurgery, Assistant Prof., 医学部(病), 助手 (50242061)
金 彪 東京大学, 医学部(病), 助手 (90231290)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1993: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Cerebral vasospasm, / Subarachnoid hemorrhage, / Smooth muscle, / Permeability, / DNA-binding dye, / Apoptosis / TUNEL法 / 膜透過性 / 細胞内カルシウム |
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| Research Abstract |
Alteration of sarcolemmal permeability was evaluated in the cerebral artery after subarachnoid hemorrhage (SAH). Significance of membrane dysfunction in the pathogenesis of chronic spasm and contribution of apoptosis were investigated in a canine model.
Permeability of the smooth muscle cell (SMC) membrane was assessed by double staining with a hydrophilic (ethidium bromide : EB) and a lipophilic (Hoechst 33342 : H33342) DNA-binding dye. Quantitative observations were made with a UV-fluorescence microscope and a UV-laser confocal microscope. Occurrence of apoptosis was studied using electrophoresis and TUNEL method. In the normal arteries, nuclel of SMC were stained with H33342 but not with EB.In the spastic arteries obtained seven days after SAH,45(]SY.+-。[)19% of SMC in the inner layr of the tunica media were stained with EB.The incidence of EB-positive cells decreased in two to four weeks (13(]SY.+-。[)5.2% and 5.0(]SY.+-.[)2.1% respectively) in parallel with amelioration of spasm. Electron and light microscopic observations revealed increased density of SMC cytoplasm with widening of the extracellular space. Necrosis was not evident. Apoptosis was not detected by the two methods. These results demonstrated that an augmentation in sarcolemmal permeability takes place during the course of chronic vasospasm, and suggest its close correlation to pathogenesis.
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