| Project/Area Number |
05671155
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
NAKASU Yoko Shiga Univ. Med. Sci., Dept. of Neurosurgery Assistant Prof., 医学部, 講師 (10135478)
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| Co-Investigator(Kenkyū-buntansha) |
MORIKAWA Shigehiro Shiga Univ. Med. Sci., Mol. Neurobiol. Center Assosiate Prof., 分子神経生物学研究センター, 助教授 (60220042)
NAKASU Satoshi Shiga Univ. Med. Sci., Dept. of Neurosurgery Assistant Prof., 医学部, 講師 (00135477)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1994: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1993: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | Chemical shift imaging / Diffusion-weighted imaging / Epilepsy / Magnetic resonance imaging / Magnetic resonance spectroscopy16FA06 : Rat / Rat / 拡散強調画像 / 核磁気共鳴 / 興奮性アミノ酸 / スペクトロスコピー / てんかん / 脳浮腫 / 扁桃核 / ラット / てんかん重積 / 辺縁系 |
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| Research Abstract |
We investigated in vivo changes in proton MR images, diffusion-weighted images (DWI) and 31P chemical shift images of the rat brain after sustained limbic seizures.
METHOD : Intraperitoneal injection of kainic acid(KA)was used to induce sustained limbic seizures in ten rats. The animals were investigated with serial T1-and T2-weighted 1H MR images (T1WI,T2WI), and DWI with 2.0 T MR (GE,Oega), beginning immediately after KA induced status. pH images were constructed from in vivo 3-D 31P MR chemical shift data.
RESULTS : DWI revealed an increase in signal intensity in the amygdala and the piriform cortices immediately after the sustained seizures, whereas T1WI and T2WI did not show changes at the time. Both DWI and T2WI showed marked increase in signal intensities in the same areas 24 hours after KA injection. The abnormal areas of on DWI were concordant with the histological distribution of neuronal pyknosis and neuropile vacuolation. The pH images spatially resolved reduced tissue pH in the bilateral temporal lobes.
CONCLUSION : DWI revealed focal abnormalities in the limbic system after sustained seizures induced by KA.These focal changes on DWI may be attributed to intracellular oedema. pH mapping showed tissue acidosis in the limbic system. These non-invasive methods will contribute in clinical evaluation and management of patients with intractable epilepsy.
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