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Analysis of tumor-suppressor gene in pediatric malignant brain tumors

Research Project

Project/Area Number 05671163
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Cerebral neurosurgery
Research InstitutionKobe University School of Medicine

Principal Investigator

KOKUNAI Takashi  Kobe University School of Medicine, Instructor of Attached Hospital, 医学部・附属病院, 講師 (30178248)

Co-Investigator(Kenkyū-buntansha) TAMAKI Norihiko  Kobe University School of Medicine, Professor, 医学部, 教授 (10030941)
Project Period (FY) 1993 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1994: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1993: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsBrain tumor / Tumor-suppressor gene / p53 gene / Nerve growth factor receptor / Differentiation / Medulloblastoma
Research Abstract

The expression of mutant p53 gene product was found in 34% mudulloblastoma, 22% ependymoma, and 47% other gliomas in various pediatric brain tumors, although it was not found in normal brain tissues including cerebellum. And also, the localization of mRNA of mutan p53 gene analyzed by in situ hybridization was the almost same localization of mutant p53 gene product. And also, the tissues with high MIB1 index were shown the high expression of mutant p53 gene product. It was correlated between the growth potential and the expression of mutant p53 gene product.
In primitive neuroectodermal tumors, nerve growth factor receptor (NGF-R) was expressed in immature neurogenic tumors, such as medulloblastoma.. We selected the subclones of tropomyosin receptor kinase (trk) -positive cells and low-affinity NGF-R-positive cells from human medullobrastoma cell line (MED-3) and examine the responsibility against NGF.Trk-positive subclone showed the induction of differentiation and the inhibition of cell growth inducd by NGF.It was suggested the role of trk gene protooncogene in differentiation of medulloblastoma. Furthermore, the prognosis in trk-positive medulloblastoma was better than that in trk-negative medulloblastoma. On the otherhand, there was no correlation between the prognosis, the period of recurrence and the responsibility against treatment and the expression of mutant p53 gene product. From the above results, it was suggested that trk protooncogene is the target gene in differentiation of medulloblastoma.

Report

(4 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] T. Kokunai: "Localization of apoptotic cells in situ of brain tumors" Brain Tumor Pathol.12. 15-21 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 穀内隆: "Glioma組織内浸潤リンパ球を用いたヒトモノクローナル抗体の作製" 神経免疫研究. 8(発表予定). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 穀内隆: "正常型p53遺伝子導入glioma細胞の特性" 神経免疫研究. 8(発表予定). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 穀内隆: "Medulloblastomaの予後に対するtrk遺伝子産物の関与" 神経免疫研究. 8(発表予定). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H. Sawa: "Growth and immunogenicity in human glioma in the SCID mice." Neurologia Medico-Chirurugica. 36(発表予定). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] S. Tatsumi: "Preparation of monoclonal aneibody against human medulloblastoma" Neurologia Medico-Chirurugica. 36(発表予定). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T. Kokunai: "Brain Tumor Research and Therapy" Springer-Verlag(印刷中), (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takashi Kokunai: "Localization of apoptotic cells in situ of brain tumors." Brain Tumor Pathology. 12-1. 15-21 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takashi Kokunai: "Preparation of human monoclonal antibody using glioma-infiltrating lymphocyte." Neuroimmunology. 8 (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takashi Kokunai: "Characterization of glioma cells transfected with wild-type p53 gene." Neuroimmunology. 8 (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takashi Kokunai: "Relation of trk proto-oncogene product in prognosis of medulloblastoma." Neuroimmunology. 8 (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hideki Sawa: "Growth and immunogenicity in human glioma in the SCID mice." Neurologia Medico-Chirurugica. 36 (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shotaro Tatsumi: "Preparation of monoclonal antibody against human medulloblastoma." Neurologia Medico-Chirurugica. 36 (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takashi Kokunai: "Expression of trk proto-oncogene product in medulloblastoma." Brain Tumor Research and Therapy. Springer-Verlag (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T. Kokunai: "Localization of apoptotic cells in situ of brain tumors." Brain Tumoy Pathol.12. 15-21 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 穀内 隆: "Glioma組織内浸潤リンパ球を用いたヒトモノクローナル抗体の作製" 神経免疫研究. 8(発表予定). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 穀内 隆: "正常型p53遺伝子導入glioma細胞の特性" 神経免疫研究. 8(発表予定). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 穀内 隆: "Medulloblastomaの予後に対するtrk遺伝子産物の関与" 神経免疫研究. 8(発表予定). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] H. Sawa: "Growth and immunoglnicity in human glioma in the SCID-hu mice." Neurologia Medico-Chirurugica. 36(発表予定). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] S. Tatsumi: "Preparation of monoclonal antibody against human medulloblastoma." Neurologia Medico-Chirurugica. 36(発表予定). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] T. Kokunai: "Brain Tumor Research and Therapy" Springer-Verlag (印刷中), (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 澤 秀樹: "脳腫瘍関連抗原(TA226)に対するヒト抗イディオタイプ抗体の作製と機能解析" 神経免疫研究. (発表予定).

    • Related Report
      1994 Annual Research Report
  • [Publications] 穀内隆: "Primitire neuroectodermal tumorsにおけるNerve growth factorの役割" 神経免疫研究. 6 (発表予定). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 穀内隆: "ヒトBリンパ球機能におけるNerve growth factorの役割" 神経免疫研究. 6 (発表予定). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Takashi Kokunai: "Expression of Nerve growth Factor Receptor by Human Primitive Neuroectodermal tumors" Neurologia medico-chirnrgica. 34 (発表予定). (1994)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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