| Project/Area Number |
05671166
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | School of Medicine, The University of Tokushima |
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Principal Investigator |
UEDA Shin The Univ.of Tokushima, School of Medicine, Associate Profeffor, 医学部, 助教授 (10093840)
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| Co-Investigator(Kenkyū-buntansha) |
NISHITANI Kasutoshi The Univ.of Tokushima, University Hospital Medidal Staff, 医学部・付属病院, 医員
SATOH Koichi The Univ.of Tokushima, University Hospital Assistant, 医学部・付属病院, 助手 (90225938)
阿川 昌仁 徳島大学, 医学部・附属病院, 助手 (70253193)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1993: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | carotid endarterectomy / endothelial regeneration / ovoid type cell / hypercholesterolemia. / endothelial regeneration |
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| Research Abstract |
Endothelial regeneration of the rabbit carotid artery was investigated after endarterectomy, which involved the removal of a 4mm length including the endothelium, internal elastic laminae and part of the muscle layr of the media. Regeneration was studied at intervals from 1 hour to 8 weeks after surgery. There were two groups, Group A consisting of Japan White rabbits fed a standard diet and Group B consisting of rabbits fed a 1% cholesterol diet for a month before the surgery and continued after surgery until the annimals were killed. In Group A,scanning electron microscopy (SEM) showed spindle type and ovoid type cells which were morphologically different from spindle type cells on the Juminal surface of the endarteretomized wall on day 3 after sugery. Endothelial regrowth 2 weeks after the sugery was confirmed. After the endothelial regrowth was completed, the ovoid type cells were localized in the low shear stress area. Transmission electron microscopy (TEM) showed the ovoid type cell consisted of pinocytic vesicles and in structure were tightly joined. Immunohistochemical findings showed the ovoid type cell stained positivery for factor, which confirmed their endothelial origin. In Group B the speed of the endothelial regrowth was not remarkably different from that in Group A,but SEM revealed enlarged spindle type cells and irregular-sized ovoid type cells. TEM showed vacuoles existed in both spindle type and ovoid type cells. Immunohistochemically the Croup B ovoid type cells stained positively for CD31, which confirmed their endothelial origin. In addition, foam cells which were stained for RAM11 migrated subendothelially, after which intimal thickening was observed.
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