| Project/Area Number |
05671207
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY |
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Principal Investigator |
TSUJI Haruo Toyama Medical and Pharmaceutical University Orthopaedics, Professor, 医学部・整形外科学, 教授 (90009449)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIHARA H Toyama Medical and Pharmaceutical University Orthopaedics Assist, Prof, 附属病院・整形外科, 助手 (30242499)
MATSUNO H Toyama Medical and Pharmaceutical University Orthopaedics Assist, Prof, 医学部・整形外科学, 助手 (00219461)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1994: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1993: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Monoclonal antibody / Intervertebral disc / Immunology / Immunohistochemistry |
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| Research Abstract |
Type II collagen existing in the hyaline cartilage and nucleus pulposus of the intervertebral disc have a specific property as an isolated antigen for immunological reactions.
In considering the mechanism of intervertebral disc degeneration, annular rupture may provide contact with Type II collagen in the nucleus pulposus and the lymphocytes in vivo. In humans, not a few patients show severe multiple intervertebral disc degeneration irrespective of their clonological ages, suggesting an immulogical background to represent disc degeneration. From the experimental results of hyaline cartilage observation, CB-11 peptide of Type II collagen is a specific fragment of immunoreactivity of T II C.In this reserch project, mamy efforts have been paid to refine the specific monoclonal antibody of CB-11 peptide (CB-11mAb). The pilot study clarified that the CB-11mAb recognized the antigen epitope of T II C from the results of immunosuppressive efects of CB-11mAb on collagen-induced arthritis in mice. Using an ABC method, serial immunohistochemical sections from 12 fresh intervertebral discs (mainly nucleus pulposus with various grade of degeneration) obtained by surgery was reacted with the CB-11mAb. However, none of positive immunoreactivity of Type II collagen was observed in degenerated nucleus pulposus.
Thus the conclusion was drawn to be negligible on T II C immunologic mechanism on the process of intervertebral disc degeneration in humans.
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