Deuterium oxide and inhalation anesthetics.
| Project/Area Number |
05671261
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Osaka University |
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Principal Investigator |
MASHIMO Takashi Osaka Univ.Medical School, Associate Professor, 医学部, 助教授 (60157188)
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| Co-Investigator(Kenkyū-buntansha) |
IKUTA Nobuo Osaka Municipal Techinical Institute, Research Chief, 有機化学科, 研究主任
YOSHIYA Ikuto Osaka Univ.Medical School, Professor, 医学部, 教授 (80028505)
幾多 信生 大阪市立工業研究所, 有機化学科, 研究主任
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1993: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Deitium Oxide / Isoflurane / MAC |
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| Research Abstract |
Anesthetics release the hydration water molecule from the interface of membranes and proteins. The structure change of water by anesthetics has been proposed to be the mechanism of anesthesia. Deuterium oxide (heavy water : D_2O) potentiates the hydration, and changes the water structure. However, the effects of D_2O on the anesthetic potency in biological system have never been reported. In this study, we gave D_2O to mouse by drinking water for 2 weeks (chronic experiment), or by peritoneal infusion of physiological saline D_2O solution (acute experiment). Both in chronic and acute experiments, D_2O decreased MAC (minimum alveolar concentration) of inhalation anesthetics (isoflurane), and increased the anesthetic potency of isoflurane. Furthermore, D_2O showed the anesthesia-like effects, such as disappearance of righting reflex and analgesia. From NMR study, we found that the anesthetic potency of alcohols correlates to the number of hydration water around alcohol molccule. The mechanisms of potentiation of anesthesia by D_2O were the followings :
1) D_2O potentiate the pharmacological efficacy of anesthetics by increasing number of hydration water molecule around anesthetics.
2) Because D_2O has the anesthetic-like effect, D_2O increases the anesthetic potency of isoflurane, additively.
3) D_2O affects anesthetic binding to membranes and proteins, and changes the distribution of anesthetics in biological system.
The present results indicate that the structure change of water molccule relates to anesthesia.
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Report
(3 results)
Research Products
(11 results)
