Urethral function and Aging

Research Project

Project/Area Number	05671303
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	Urology
Research Institution	TOKYO MEDICAL AND DENTAL UNIVERSITY
Principal Investigator	MORITA Takashi TOKYO MEDICAL AND DENTAL UNIVERSITY.ASSOCIATE PROFESSOR, 医学部, 助教授 (10006819)
Project Period (FY)	1993 – 1994
Project Status	Completed (Fiscal Year 1994)
Budget Amount *help	¥2,000,000 (Direct Cost: ¥2,000,000) Fiscal Year 1994: ¥1,000,000 (Direct Cost: ¥1,000,000) Fiscal Year 1993: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywords	urethra / alpha-receptor / endothelin receptor / sex hormone / 膀胱エンドセリン受容体 / 尿失禁 / 尿道の受容体 / 尿道平滑筋 / α受容体 / NO(一酸化窒素) / 加齢
Research Abstract	Urethral function and aging Takashi Morita M.D. Department of Urology.Tokyo Medical and Dental University The effects of age on autonomic receptors (alpha_1-R,alpha_2-R) and endotherin-1 receptor (ET-1-R) densities were studied using ^<125>I-ET-1, ^3H-prazosin (PZ) and ^3H-yohimbin (YOH) as ligands. Cell membrane homogenates of male Wistar rat urethra. 1,2,3,7 and 15 weeks old, were used in this experiment. Saturation experiments revealed that there were significant amounts of saturable high affinity binding sites for ^3H-PZ,^3H-YOH and ^<125>I-ET-1 in rat urethra from all age groups. The Bmax values of ^<125>I-ET-1 were higher in the 1 and 2 week-old groups than in the 3,7 and 15 week-old groups. On the other hand, the Bmax values. of ^3H-PZ were higher in older groups than in younger groups and the Bmax values of ^3H-YOH were not different in all groups. These findings suggest that there are age differences in autonomic receptors in rat urethra. The age differences may be related to sex hormones.