| Project/Area Number |
05671308
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Urology
|
|---|
| Research Institution | FUKUI MEDICAL SCHOOL |
|---|
Principal Investigator |
OKADA Kenichirou Fukui Medical School, Fuculty of Medicine, Professor Department of Urology, 医学部, 教授 (60026838)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
FUJITA Tomohiro Fukui Medical School, Faculty of Medicine, Research Associate Department of Urol, 医学部, 助手 (60240785)
AKINO Hironobu Fukui Medical School, Faculty of Medicine, Research Associate Department of Urol, 医学部, 助手 (90159335)
|
|---|
| Project Period (FY) |
1993 – 1994
|
| Project Status |
Completed (Fiscal Year 1994)
|
| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1993: ¥1,800,000 (Direct Cost: ¥1,800,000)
|
|---|
| Keywords | Epidermal growth facter / Prostate / Immunohistochemical / aging |
|---|
| Research Abstract |
To clarify the presence of epidermal growth facter (EGF) in the prostate, we investigated a total of 65 prostatic tissues : normal prostate (N=11), benign prostaic hyperplasia (N=42), prostatic carcinoma (N=12).
Tissues were fixed in neutral buffered formalin and embedded in paraffin. Deparaffinized and rehydrated 3mum sections were treated with 3% H_2O_2 in pure methanol for 30 minutes. Incubate specimens with blocking serum ini PBS,20 minutes, to suppress non-specific binding of IgG.Incubate tissue section with 75mul of PBS-BSA containing diluted antibody OSI primary Antibody or OSI negative control antibody for overnight at 4 ﾟC.Incubate with OSI biotinylated secondary IgG for 30 minutes. Incubate with OSI ABC reagent for 30 minutes. Rinse in 1% Triton X-100-PBS for 30 sections. Incubate in DAB solution for 7 minutes. Counterstain in hematoxylin solution for 10 sections.
All epithelial cells and stromal elements had undetectuble levels of EGF expression.
The results suggest that EGF is not the major growth facter of prostate.
|
