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Gene therapy for urological cancer by IFN-gamma gene transduction

Research Project

Project/Area Number 05671313
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Urology
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

HASHIMURA Takayuki  KYOTO UNIVERSITY,Faculty of Medicine, Associate Professor, 医学部, 講師 (40189478)

Co-Investigator(Kenkyū-buntansha) MIZUTANI Yoichi  KYOTO UNIVERSITY,Faculty of Medicine, Instructor, 医学部, 助手 (10243031)
栗林 景容  京都大学, 医学部, 助手 (10064578)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1994: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1993: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsGene therapy / Bladder cancer / Interferon-gamma / Retro virus / MBT-2 / Class I MHC / CD8(+) T-cell / 泌尿器科癌 / リポゾーム / ヒト膀胱癌 / 免疫療法 / 腎癌
Research Abstract

Mouse IFN-gamma gene was transfered into mouse bladder cancer cells (MBT2) by retroviral vector and the IFN-gamma producing cell line, MBT2 (Kgamma), was established. Tumor formations were not observed in mice injected subcutaneouslly with 1.5x105 MBT2 (Kgamma). Furthermore, mice that rejected MBT2 (Kgamma) became highly resistant to a subsequent challenge with parental MBT2, but not to C3H8.2 fibrosarcoma cells. The spleen cells derived from the immunized mice showed the populations of CD8+ effectorT cells against MBT2, which suggests the induction of specific antitumor immunity. For the clinical application of the cytokine gene therapy, the experiment of in vivo direct IFN-gamma gene transfer to MBT2 tumors was performed. After the subcutaneous inoculation of 1.5x105 MBT2 cells into mice, virus culture supernatant containing IFN-gamma gene, psi2 (Kgamma) or psi2 (Mugamma), was injected into the same tumor site once a day for 3 days. In about 40% of mice of treatment of groups with psi2 (Kgamma) or psi2 (Mugamma), tumor formations were not observed. Tumor free survivals and actuarial survivals in the both treatment groups were significantly longer than those of control group. These results showed the possibility of in vivo direct IFN-gamma gene transfer into tumors and were encouraging for the promising execution of tumor cell-targeted IFN-gamma gene therapy against human bladder cancer.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 橋村孝幸 他: "膀胱癌細胞へのin vivoにおけるInterferon-γ遺伝子導入による遺伝子治療" 泌尿器科紀要. 43. 809-813 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kenji Kawabata et al: "A physiological role of interferon-β derived from tunor" Cancer Letter. 113. 159-164 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Hiura et al: "Induction of specific Anti-tumor Immunity by Interferon-γ Gene-transfered Murine Bladder Carcinoma MBT-2" Folia Biologica. 40. 49-61 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 橋村孝幸 他: "膀胱癌細胞へのIFN-γ遺伝子導入による癌免疫療法" 泌尿器科紀要. 39. 1205-1208 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takayuki Hashimura et al.: "Gene therapy by in vivo IFN-gamma gene transfer to murine bladder tumor" Acta Urol.Jpn.43. 809-813 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kenji Kawabata et al.: "A physiological role of interferon-beta derived from tumor" Cancer Letter. 113. 159-164 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Hiura et al.: "Induction of Specific Anti-tumor Immunity by Interferon-gamma Gene-Transferred Murine Bladder Carcinoma MBT-2" Folia Biologica. 40. 49-61 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takayuki Hashimura et al.: "Cancer immunotherapy by murine bladder cancer cells transfected with mouse interferon-gamma gene" Acta Urol.Jpn.39. 1205-1208 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 橋村孝幸: "膀胱癌細胞へのIFN-γ遺伝子導入による癌免疫療法" 泌尿器科紀要. 39. 1205-1208 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Masuru Hiura etal.: "Induction of Specific Anti-tumor Immunity by Interferone-γ Gene-transferred Murine Bladder Carcinoma MBT-2" Folia Biologica. 40. 49-61 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 橋村孝幸: "膀胱癌細胞へのIFN-γ遺伝子導入による癌免疫療法" 泌尿器科紀要. 39. 1205-1208 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.HIURA: "Induction of specific Anti-tumor Immunity by Interferon-γ Gene-transferred Murine Bladder Carcmoma MBT-2" Folia Biologica. 40(in press). (1994)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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