| Project/Area Number |
05671321
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Urology
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| Research Institution | Ehime University |
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Principal Investigator |
YOKOYAMA Masayoshi Ehime University School of Medicine University Hospital Lecturer, 医学部・附属病院, 講師 (50116993)
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| Co-Investigator(Kenkyū-buntansha) |
ISEDA Tokuhiro Ehime University School of Medicine Assistant, 医学部, 助手 (50260386)
HAMADA Hitoshi Ehime University School of Medicine University Hospital Assistant, 医学部・附属病院, 助手 (30243794)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1994: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1993: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | bladder cancer / serum marker / flow cytometry / EH14 / monoclonal antibody / ELISA / 移行上皮癌 / 腫瘍マーカー |
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| Research Abstract |
A specific and sensitive monoclonal antibody against transitional cell cancer (TCC) may be useful for early detection of the tumor and precise monitoring of the treatment. We have developed a monoclonal antibody (EH14) against TCC.In this program we tried to purify the antigen recognized by EH14. We also compared the antigen concentration in the serum and the urine of patients with TCC to healthy controls. In order to evaluate the difference of the characteristics between the EH14 positive and negative cells, the TCC cells were exmined by flow cytometry. In addition, we are making cDNA libraries from TCC cell lines to identify the EH14 antigen gene.
Using an affinity coloumn EH 14 antigen was partially purified and the amino acid sequence is to be determined. Using a sandwich ELISA system, the serum and the urine concentrations of EH14 antigen in patients with TCC were significantly higher than tose in healthy controls. The patients with lymph node metastases had higher concentration of the serum antigen titer than tose without lymph node metastases although not statistically significant. Flow cytometric evaluation showed that the patterns of DNA ploidy and EH14 staining varied from patient to patient. The statistical analysis will be done after the accumulation of cases examined.
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