• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Comparative study of erdometrial carciroma between Japanese women and American women

Research Project

Project/Area Number 05671347
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionTohoku University

Principal Investigator

SATO Shinji  Tohoku University Dept. of OB/GYN,Associate Prof., 医学部, 講師 (10142960)

Co-Investigator(Kenkyū-buntansha) SASANO Hironobu  Tohoku University Dept. of Pathology, Instructor, 医学部, 助手 (50187142)
FUJITA Nobuhiro  Tohoku University Dept. of OB/GYN,Instructor, 医学部附属病院, 助手 (20250748)
ITO Kiyoshi  Tohoku University Dept. of OB/GYN,Instructor, 医学部附属病院, 助手 (70241594)
八重樫 伸生  東北大学, 医学部附属病院(産婦人科学), 助手 (00241597)
田勢 亨  東北大学, 医学部附属病院(産婦人科学), 講師 (50179701)
Project Period (FY) 1993 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1994: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1993: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsP450 aromatase / Endometrial carcinoma / lmmunohistochemistry / 癌遺伝子 / PCR-RFLP法
Research Abstract

The expression of P450 aromatase and other steroidogenic enzymes were evaluated in 42 endometrioid endometrial carcinomas, 23 endometrial hyperplasias, and 7 normal endometrial specimens. These findings were correlated with clinicopathological findings to elucidate the possible biological significance of in situ estrogen production in the development of human endometrial carcinoma. Only weak aromatase immunoreactivity was observed in vascular walls and myometrial cells. In contrast, strong aromatase stromal immunoreactivity was observed in 28 of 42(66.7%)endometrial carcinomas. However, no stromal immunoreactivity was seen in normal or hyperplastic endometrial specimens. Immunoreactivity in the carcinoma stromal cells was significantly increased at sites of invasion. These aromatasepositive cells were immunohistochemically negative for other steroidogenic enzymes involved in estrogen biosynthesis. In situ hybridization studies revealed aromatase mRNA hybridization signals in stromal ce … More lls but not in carcinoma cells. The distribution of aromatase mRNA correlated well with the immunohistochemical localization of aromatase anzyme. Quantitation of aromatase activity demonstrated 8.75 <minus-plus> 2.75 pmol/hour/mg of protein for endo-metrial carcinomas (22 specimens) and 0.98 <minus-plus> 1.95 pmol/hour/mg of protein for normal endometrial specimens(4 specimens). Aromatase activity was found in both estrogen receptor-positive and -negative endometrial carcinoma. Aromatase did not vary with respect to the menopausal status of patients with endometrial carcinoma. These results suggest that estrogen is produced in situ in endometrial carcinoma but not in benign endometrial lesions. Such locally synthesized estrogen may act on carcinoma cells in a paracrine fashion to promote tumor growth. Additional inverstigations are necessary, but increased aromatase expression in the stromal cells of endometrial carcinoma may therefore play an important role in the development of human endometrioid endometrial carcin Less

Report

(4 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • Research Products

    (5 results)

All Other

All Publications (5 results)

  • [Publications] Watanabe K.,Sata S.: "Aromatase in human endometrial carcinoma and hyperplasia" American Journol of Pathology. 146. 491-500 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Watanabe K, Sato S et al: "Aromatase in human endometrial carcinoma and hyperplasia" American J.of Pathology. 146. 471-500 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] WatanabeK, Sato S et al: "Aromatase in human endometrial carcinoma and hyperplasia" American Journal of Pathology. 146. 491-500 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ito K,Sato S,et al: "Rrognostic significance of p53 overefpression in enclometrial cancer" Cancer Research. 54. 4667-4670 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 佐藤信二: "癌関連遺伝子異常、細胞増殖能および細胞外マトリックスの検索による子宮体癌・子宮内膜増殖症の生物学的悪性度の評価" 日本産科婦人科学会雑誌. 45. 776-788 (1993)

    • Related Report
      1993 Annual Research Report

URL: 

Published: 1993-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi