| Project/Area Number |
05671362
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Nagoya University |
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Principal Investigator |
ASADA Yoshimasa (1995) Nagoya Univ.School of Medicine, Associate Prof., 医学部, 講師 (70231884)
古井 憲司 (1994) 名古屋大学, 医学部, 助手 (10261223)
福垣 洋行 (1993) 名古屋大学, 医学部, 助手 (10252248)
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| Co-Investigator(Kenkyū-buntansha) |
SUGANUMA Nobuhiko Nagoya Univ.School of Medicine, Associate Prof., 医学部, 助教授 (30179113)
KONDO Ikuyo Nagoya Univ.School of Medicine, Assistant Prof., 医学部, 助手 (40215447)
原田 賢彦 名古屋大学, 医学部, 助手
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| Project Period (FY) |
1993 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1994: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1993: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | Inhibin / Activin / Follistatin / Gene expression / Granulosa cell / Northern blotting / ノーザンプロット / メッセンジャーRNA / PCR / RT-PCR |
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| Research Abstract |
To analyze direct effects of estrogen on gene expressions of inhibin subunits in vitro, the mRNA levels of inhibin alpha- and betaA-subunits were measured in rat granulosa cells cultured with FSH or estradiol. Through the culture process of the granulosa cells with serum-free medium, both the alpha- and betaA subunit mRNAs decreased, which were partially rescued by addition of FSH to the culture medium. To examine whether these FSH effects is mediated via estrogen production, estradiol and/or tamoxifen, one of anti-estrogenic drugs, were added to the cultured granulosa cells. After 36-hour culture with estradiol, the inhibin alpha-subunit decreased whereas the inhibin betaA-subunit increased, with dose-responsive manner. Tamoxifen showed a completely reversed effects to estradiol, and combination of estradiol and tamoxifen resulted similar levels of inhibin-alpha and -betaA mRNAs with control. These results indicated that estrogen would have a certain pathway to affect gene expressions of the inhibin subunits in the rat granulosa cells, and may regulate the production of inhibin and activin through paracrine system.
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